M6320

Monoclonal Anti-MAGE-1

~2 mg/mL, clone MA454, purified immunoglobulin, buffered aqueous solution

• Sinonimo/i: Anti-Melanoma-associatiated Antigen 1
• Numero MDL: MFCD03096998
• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Coniugato: unconjugated
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: MA454, monoclonal
• Stato: buffered aqueous solution
• PM: antigen ~46 kDa
• Reattività contro le specie: human
• Confezionamento: antibody small pack of 25 μL
• Concentrazione: ~2 mg/mL
• tecniche: immunohistochemistry: suitable; indirect ELISA: suitable; western blot: 5-10 μg/mL using total cell extract of SKML37 cells
• Isotipo: IgG1
• N° accesso UniProt: P43355
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... MAGEA1(4100)

Descrizione

Descrizione generale

Melanoma-associatiated antigen 1 (MAGE-1) is part of the CG (cancer-germline) group of genes and is a tumor-specific antigen.[1][2] MAGE-1 is a member of the MAGE-A family. MAGE-1 gene is localized on human chromosome Xq28.[3] Monoclonal Anti-MAGE-1 (mouse IgG1 isotype) is derived from the hybridoma MA454 produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from mice immunized with human recombinant MAGE-1.

Specificità

The antibody does not recognize MAGE-2 and MAGE-3.[4][5]

Immunogeno

recombinant human MAGE-1.

Applicazioni

Monoclonal Anti-MAGE-1 antibody produced in mouse has been used in:

• ELISA
• immunoblotting
• immunohistochemistry

Azioni biochim/fisiol

The melanoma antigen genes (MAGE) might play a key role in neural development and apoptosis. Expression of melanoma-associated antigen 1 (MAGE-1) in neoplastic cells depends on the binding of the transcription factor Ets to non-methylated cis regulatory sequences, located in its promoter. MAGE-1 expression is mediatedby the B and B′ domains of MAGE-A1 gene. Expression of MAGE-1A was induced in non MAGE-A1 expressing tumor cells once treated with demethylating reagent, 5-aza-2-(5DC).[6] Melanoma-associatiated antigen 1 (MAGE-1) has been shown to be expressed in different types of cancers.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)