Catalog numbers L1668 and L8902 are suitable for cell culture assays and cell stimulation. The end user needs to design and optimize the assays. These products have not been specifically tested for intracellular cytokine staining assays. It is recommended to review the literature for further information.
L8629
Lectin from Phaseolus vulgaris (red kidney bean)
Erythroagglutinin PHA-E
Sinonimo/i:
Phaseolus vulgaris agglutinin, PHA
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5 MG
CHF 247.00
CHF 247.00
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5 MG
CHF 247.00
About This Item
Codice UNSPSC:
12352202
NACRES:
NA.32
CHF 247.00
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Prodotti consigliati
Livello qualitativo
Stato
salt-free, lyophilized powder
Potenza
<10 μg per mL agglutination activity (with erythryocytes)
Composizione
Protein, ≥80% biuret
Temperatura di conservazione
2-8°C
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Categorie correlate
Azioni biochim/fisiol
PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.
Qualità
Tested for leucoagglutination.
Nota sulla preparazione
Purified by a modification of the method of Leavitt.[1]
Risultati analitici
Agglutination activity is expressed in μg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of either human erythrocytes (2% in phosphate buffered saline, pH 6.8) or human leukocytes (107 per mL in saline) after 1 hr incubation at 25 °C.
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
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Articoli
Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.
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Which PHA is more suitable for cell stimulation and intracellular cytokine staining assays?
1 answer-
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What do the designations -P, -L, -M, and -E represent in the names of PHA products?
1 answer-
Generally, PHA-P or PHA-L is utilized to stimulate lymphocytes, although PHA-M will also work, but not to the same degree. The most active form is PHA-L (the mitogenic concentration is < 5μg/ml). PHA, or Phytohemagglutinin, comprises two molecular species:
1. Erythroagglutinin (PHA-E), which demonstrates low mitogenic activity and high erythroagglutinating activity.
2. Leucoagglutinin (PHA-L), which possesses high mitogenic and leucoagglutinating activity, accompanied by very low erythroagglutinating activity.
PHA-P represents the protein form of PHA before the erythroagglutinin and leucoagglutinin components are separated and purified. PHA-M refers to the mucoprotein form of Phytohemagglutinin.
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