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L5142

Sigma-Aldrich

Lectin from Triticum vulgaris (wheat)

biotin conjugate, lyophilized powder

Sinonimo/i:

WGA, Wheat germ agglutinin

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1 MG
CHF 71.50
5 MG
CHF 274.00

CHF 71.50

Spedizione prevista il29 maggio 2025

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1 MG
CHF 71.50
5 MG
CHF 274.00

About This Item

Codice UNSPSC:
12352202
eCl@ss:
34058011
NACRES:
NA.32

CHF 71.50

Spedizione prevista il29 maggio 2025

Coniugato

biotin conjugate

Livello qualitativo

200

Stato

lyophilized powder

Composizione

Protein, ≥80% E1%/280

Grado di funzionalizzazione

2-4 mol biotin per mol protein

Temperatura di conservazione

2-8°C

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Descrizione generale

Wheat germ lectin (WGA) from Triticum vulgaris exists as a homodimer and has multiple N-acetyl glucosamine (GlcNAc) binding sites. It is resistant to protein denaturation agents and is used for the detection of O-GlcNAc proteins.[1] WGA binding to N-glycolylneuraminic acid (Neu5Ac) may modulate pro-inflammatory immune response.[2]

Applicazioni

Lectin from Triticum vulgaris (wheat) has been used:
  • in lectin binding assay of samples from intrauterine stages of filarial parasites[3]
  • in the histological studies of mice myocytes[4]
  • for the generation bead-biotin-WGA complex with Dynabeads® M-280 Streptavidin magnetic beads for lectin magnetic separation of Toxoplasma gondii oocytes from water samples[5]

Azioni biochim/fisiol

WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.

Confezionamento

Package size based on protein content.

Stato fisico

Contains sodium citrate

Nota sulla preparazione

Conjugates are prepared from affinity purified lectin.

Note legali

Dynabeads is a registered trademark of Invitrogen Dynal AS Corp

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

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Certificati d'analisi (COA)

Lot/Batch Number
0000381539
0000381540
0000381540
0000381539
0000379442

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Evaluation of bi-ventricular coronary flow patterns using high-frequency ultrasound in mice with transverse aortic constriction
Wu J, et al.
Ultrasound in Medicine & Biology, 39(11), 2053-2065 (2013)
The dietary intake of wheat and other cereal grains and their role in inflammation
De PK and Pruimboom L
Nutrients, 5(3), 771-787 (2013)
A flow cytometry based method for studying embryogenesis and immune reactivity to embryogenic stages in filarial parasites
Sahu BR, et al.
Filaria Journal, 4(1), 11-11 (2005)
R E Morris et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 40(5), 711-721 (1992-05-01)
We demonstrate here that the intracellular routing of biotinylated ligands was not affected by the attachment of streptavidin gold colloids so long as the electron-dense marker was added after the biotinyl ligand-receptor interaction had occurred. The binding, internalization, and intracellular
Candida Fagundes Teixeira et al.
PLoS neglected tropical diseases, 1(2), e73-e73 (2007-12-07)
Diagnosis of intestinal schistosomiasis in low endemic areas is a problem because often control measures have reduced egg burdens in feces to below the detection limits of classical coproparasitological methods. Evaluation of molecular methods is hindered by the absence of
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