I2533
Anti-phospho-Insulin Receptor Substrate-1 (pSer312) antibody produced in rabbit
affinity isolated antibody, buffered aqueous glycerol solution
Sinonimo/i:
Anti-phospho-IRS-1(pSer312)
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About This Item
Prodotti consigliati
Origine biologica
rabbit
Livello qualitativo
Coniugato
unconjugated
Forma dell’anticorpo
affinity isolated antibody
Tipo di anticorpo
primary antibodies
Clone
polyclonal
Stato
buffered aqueous glycerol solution
Reattività contro le specie
human
tecniche
western blot: 1:1000 using stimulated CHO cell line
N° accesso UniProt
Condizioni di spedizione
wet ice
Temperatura di conservazione
−20°C
modifica post-traduzionali bersaglio
phosphorylation (pSer312)
Informazioni sul gene
human ... IRS1(3667)
mouse ... Irs1(16367)
rat ... Irs1(25467)
Descrizione generale
Insulin receptor substrate-1 (IRS-1) is among the four IRS proteins (IRS-1-IRS-4) that mediate signalling by insulin receptor (IR) and insulin-like growth factor (IGF) receptor. IRS-1 has a pleckstrin homology domain and a phosphotyrosine-binding domain. Phosphorylation of the Tyr1179 residue in the SH-1 negatively regulates insulin. IRS-1 has the abililty to translocate to the nucleus, the mechanism of which is not very clear. Estrogen receptors (ER) α and β have a strong affinity to IRS-1, an important interaction in breast cancers and medulloblastomas. The expression of IRS-1 in prostate cancer is associated with lower tumor cell invasiveness. Along with ER α, IRS-1 also modulates transcriptional activity involved in the development of breast and prostate cancer. The signalling pathway involving IGF-IR-IRS-1 in cells expressing JCV T-antigen has been reported to affect the proliferation, survival and DNA repair in the cells. Phosphorylation of IRS-1 at Ser312 and Ser616 results in the negative regulation of insulin signalling in humans
Anti-phospho-IRS-1 (pSer312) specifically recognizes human IRS-1 (pSer312). It may react with mouse and rat (100% homology) IRS-1.
Anti-phospho-IRS-1 (pSer312) specifically recognizes human IRS-1 (pSer312). It may react with mouse and rat (100% homology) IRS-1.
Immunogeno
synthetic phosphopeptide derived from the region of IRS-1 that contains serine 312. This sequence is 100% homologous with mouse and rat IRS-1.
Applicazioni
Anti-phospho-IRS-1 (pSer312) antibody may be used for detection by immunoblotting, in stimulated CHO cell line expressing human insulin receptor, at a working dilution of 1:1000.
Stato fisico
Solution in Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide.
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Vincent Aguirre et al.
The Journal of biological chemistry, 277(2), 1531-1537 (2001-10-19)
Serine phosphorylation of insulin receptor substrate-1 (IRS-1) inhibits insulin signal transduction in a variety of cell backgrounds, which might contribute to peripheral insulin resistance. However, because of the large number of potential phosphorylation sites, the mechanism of inhibition has been
Joanna Trojanek et al.
Journal of cellular physiology, 206(1), 35-46 (2005-06-21)
JC polyomavirus (JCV), which infects 90% of the human population, is detectable in human tumors. Its early protein, JCV T-antigen, transforms cells in vitro and is tumorigenic in experimental animals. Although T-antigen-mediated transformation involves genetic alterations of the affected cells
Sindhu Saraswathy et al.
Experimental eye research, 196, 108064-108064 (2020-05-23)
This work sought to compare aqueous angiographic segmental patterns with bead-based methods which directly visualize segmental trabecular meshwork (TM) tracer trapping. Additionally, segmental protein expression differences between aqueous angiographic-derived low- and high-outflow human TM regions were evaluated. Post-mortem human eyes
Catia Morelli et al.
Oncogene, 23(45), 7517-7526 (2004-08-20)
Insulin receptor substrate 1 (IRS-1) is a major signaling molecule activated by the insulin and insulin-like growth factor I receptors. Recent data obtained in different cell models suggested that in addition to its conventional role as a cytoplasmic signal transducer
M G Myers et al.
Endocrinology, 132(4), 1421-1430 (1993-04-01)
IRS-1 is a unique cytosolic protein that becomes tyrosine phosphorylated during insulin stimulation of intact cells and immediately associates with the phosphatidylinositol 3'-kinase (PtdIns 3'-kinase). The insulin-like growth factor-I (IGF-I) receptor also mediated the tyrosine phosphorylation of IRS-1 and increased
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