I2519
Monoclonal Anti-Interleukin-8 antibody produced in mouse
clone 6217, purified immunoglobulin, lyophilized powder
Sinonimo/i:
Anti-CXCL8
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About This Item
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Coniugato
unconjugated
Forma dell’anticorpo
purified immunoglobulin
Tipo di anticorpo
primary antibodies
Clone
6217, monoclonal
Forma fisica
lyophilized powder
Reattività contro le specie
human
tecniche
capture ELISA: suitable
flow cytometry: suitable
immunocytochemistry: 25 μg/mL using using calcium ionophore- and PMA-stimulated human peripheral blood mononuclear cells
neutralization: suitable
western blot: 1-2 μg/mL
Isotipo
IgG1
Temperatura di conservazione
−20°C
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... IL8(3576)
Descrizione generale
Interleukin-8 or CXCL8 (IL-8) is a proinflammatory chemokine that belongs to the C-X-C family. Monocytes and macrophages are the primary sources of IL-8 but it is also secreted by most nucleated cells. IL-8 binds to two G protein-coupled receptors, CXCR1 and CXCR2 and leads to the downstream activation of PI3K, MAPK, PKC, STAT3 and FAK pathways. IL-8 is responsible for the recruitment monocytes and neutrophils that results in a prolonged acute inflammation. The expression of IL-8 is influenced by inflammatory signals, chemical and environmental stress and hormones. Deregulation of IL-8 signaling has been observed in atherosclerosis, cardiovascular disease and solid tumors of bladder, prostate, ovary, glial cells and pancreas. Monoclonal Anti-Interleukin-8 antibody recognizes human IL-8. By immunoblotting, the antibody shows 100% cross reactivity with recombinant porcine IL-8, and no cross reactivity with recombinant rat CINC-2α.
Immunogeno
purified, E. coli-derived, recombinant human IL-8.
Applicazioni
Monoclonal Anti-Interleukin-8 antibody may be used for neutralization assays; ND50 is 0.08-0.4 μg/ml. The antibody may be used for immunoblotting at a working concentration of 1-2 μg/ml. For ELISA a working concentration of 0.5 μg/ml may be used. Antibody concentration of 25 μg/ml may be used for immunocytochemistry using stimulated human peripheral blood mononuclear cells. For flow cytometry, 10 μL of 25 μg/ml stock antibody solution may be used for 1-5 X 105 cells.
Stato fisico
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates.
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
Certificati d'analisi (COA)
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I clienti hanno visto anche
C Knall et al.
Proceedings of the National Academy of Sciences of the United States of America, 94(7), 3052-3057 (1997-04-01)
Chemoattractants and chemokines, such as interleukin 8 (IL-8), are defined by their ability to induce directed cell migration of responsive cells. The signal transduction pathway(s) leading to cell migration remain ill defined. We demonstrate that phosphatidylinositol-3-kinase (PI3K) activity, as determined
Moira L Aitken et al.
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The role of inflammation in airway epithelial cells and its regulation are important in several respiratory diseases. When disease is present, the barrier between the pulmonary circulation and the airway epithelium is damaged, allowing serum proteins to enter the airways.
C Knall et al.
The Journal of biological chemistry, 271(5), 2832-2838 (1996-02-02)
Interleukin-8 (IL-8), the prototypic member of the CXC subfamily of chemokines, induces in neutrophils chemotaxis, the respiratory burst, granule release, and increased cell adhesion. The IL-8 receptor is a seven-transmembrane spanning receptor coupled to specific heterotrimeric G proteins including Gi
Daniel J Brat et al.
Neuro-oncology, 7(2), 122-133 (2005-04-16)
Interleukin-8 (IL-8, or CXCL8), which is a chemokine with a defining CXC amino acid motif that was initially characterized for its leukocyte chemotactic activity, is now known to possess tumorigenic and proangiogenic properties as well. In human gliomas, IL-8 is
Stavros Apostolakis et al.
Cardiovascular research, 84(3), 353-360 (2009-07-21)
Since the establishment of the inflammatory basis of atherosclerosis, several pro- or anti-inflammatory agents have been examined as potential mediators of the biochemical pathways of lesion formation. Interleukin (IL)-8 was first characterized in 1987. Since then, knowledge regarding its role
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