I2393

Interleukin-1β from rat

IL-1β, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Sinonimo/i: IL-1β, Interleukin-1β from rat, Lymphocyte activating factor
• Numero MDL: MFCD01094098
• Codice UNSPSC: 12352202
• NACRES: NA.77

Proprietà

• Origine biologica: rat
• Livello qualitativo: 100
• Ricombinante: expressed in E. coli
• Saggio: ≥98% (SDS-PAGE and HPLC)
• Forma fisica: lyophilized powder
• Potenza: 0.01-0.1 ng/mL ED₅₀/EC₅₀
• PM: 17.3 kDa
• Confezionamento: pkg of 10 and 50 μg
• tecniche: cell culture | mammalian: suitable
• Impurezze: <0.1 EU/μg
• N° accesso UniProt: Q63264
• Temperatura di conservazione: −20°C
• Informazioni sul gene: rat ... Il1b(24494)

Descrizione

Descrizione generale

Interleukin-1 (IL-1), originally known as Lymphocyte Activating Factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin-2.¹ IL-1 is primarily released from stimulated macrophages and monocytes, but is also released from several other cell types,² and is thought to play a key role in inflammatory and immune responses.³ Other synonyms for IL-1 include: Endogenous Pyrogen (EP), Mitogenic Protein (MP), Helper Peak-1 (HP-1), T Cell Replacing Factor III (TRF III or TRF_H), B cell Activating Factor (BAF) and B Cell Differentiation Factor (BDF).⁴ The two closely related agents, Interleukin-1α (IL-1α) and Interleukin-1β (IL-1β) bind to the same cell surface receptor, elicit nearly identical biological responses and yet share 25% homology in their amino acid sequence. Recombinant rat IL-1β is a 17.3 kDa protein containing 153 amino acid residues.

Stato fisico

Lyophilized from a 0.2 μm filtered buffered solution.

Risultati analitici

The biological activity is measured in a cell proliferation assay using murine D10S cells.

Citazione

1. Gery, I., et al., J. Exp. Med., 136, 128 (1972).
2. Oppenheim, J. J., et al., Immunol. Today, 7, 45 (1986).
3. Durum, S. K., et al., Annu. Rev. Immunol., 3, 263 (1985).
4. Aarden, L., et al., J. Immunol., 123, 2928 (1979).
5. Symons, J., et al., Lymphokines and Interferons, A Practical Approach, Clemens, M., et al., (eds.), IRL Press, Oxford (1987).

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)