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H2153

HABA/Avidin Reagent

lyophilized powder

Sinonimo/i:

HABA Reagent

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Voi/SKUDisponibilitàPrezzo
1 vial
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CHF 149.00

Informazioni su questo articolo

NACRES:
NA.46
UNSPSC Code:
12352203
Form:
lyophilized powder

CHF 149.00

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form

lyophilized powder

Quality Segment

usage

sufficient for ≤10 assays

color

orange

suitability

suitable for spectrophotometric determination of biotin and biotin conjugates in a wide variety of samples

storage temp.

2-8°C

General description

4-Hydroxyazobenzene-2-carboxylic acid (HABA) is an avidin-binding dye that can be stoichiometrically displaced by biotin. The subsequent change in absorption at A500 provides a basis for spectrophotometric determination of biotin levels in biological samples.
HABA/Avidin Reagent is specific for chromogenic applications that depend upon quantification of biotin.

Application

HABA/Avidin Reagent is suitable for spectrophotometric determination of biotin and biotin conjugates in a wide variety of samples.
Reagent is capable of detecting biotin at μMolar concentrations (i.e., 1 nmole per ml). Typically used to quantitate biotin in biotinylated compounds.

Preparation Note

Prepares a ready-to-use HABA/avidin solution in buffered saline, pH approx. 7.3, with preservative when reconstituted with 10 ml deionized water.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Codici di pericolo

H412

Avvertenze di sicurezza

P273 - P501

Hazard Classifications

Aquatic Chronic 3

Classe di stoccaggio

13 - Non Combustible Solids

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Certificati d'analisi (COA)

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Questions

1–2 of 2 Questions  

  1. I would like to know how many assays can be done with a VIAL KIT and how much material it contains,

    1 answer

    1. This product is sufficient for ≤10 assays. When reconstituted with 10 ml deionized water, each vial will yield a solution with a pH of approximately 7.3, containing the following components: 0.3 mM 4-Hydroxyazobenzene-2-carboxylic acid (HABA), 0.45 mg/ml avidin, 0.3 M sodium chloride (NaCl), 0.01 M N-[2-Hydroxyethyl]piperazine-NN-[2-ethanesulfonic acid] (HEPES), a buffer with a pKa of 7.5, 0.01 M magnesium chloride (MgCl2), and 0.02% sodium azide as a preservative.

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  2. how much HABA is in the vial

    1 answer

    1. When reconstituted to 10mL, the vial will contain 0.3 mM HABA. Based on the molecular weight of HABA, 242.23 gmol, the calculated mass per vial is 726.69 ug. Please see the link below to review additional informaition available in the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/378/451/h2153dat.pdf

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