GE25-8010-65
CyDye™ DIGE Fluor Minimal Labeling Kit
Cytiva 25-8010-65, pack of 5 nmol
About This Item
Prodotti consigliati
Forma fisica
solid
Durata
8‑12 mo. (for unreconstituted dye)
Caratteristiche
wetted part: no
Confezionamento
pack of 5 nmol
Produttore/marchio commerciale
Cytiva 25-8010-65
Condizioni di stoccaggio
protect from light
Colore
blue
red
yellow
Yellow, Red, Blue
Solubilità
cold water: soluble (Soluble in cold water and hot water)
soluble (in cold and hot water)
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
Categorie correlate
Descrizione generale
CyDye™ DIGE fluors are available as minimal and saturation labeling dyes. The minimal dyes are intended for general 2-D application use where sufficient amounts of sample are available. The saturation dyes from the Scarce Sample Labeling Kit are designed to be used for applications where only small amounts of sample are available, for example in Laser Capture Microdissection. In total, three minimal dyes and two saturation dyes are available.
CyDye™ DIGE fluors are exceptional dyes for multicolor analysis, offering bright and intense colors with narrow excitation and emission bands. The fluors are spectrally distinct, making them highly suitable for multicolor detection. CyDye™ DIGE fluors utilize these benefits but are also size- and charge-matched specifically for 2-D DIGE using Ettan™ DIGE system.
The dyes are dissolved in Dimethylformamide (DMF) and the minimal labeling reaction is quenched with L-Lysine
Caratteristiche e vantaggi
- Allow detection of up to three prelabeled protein samples and standards on the same 2-D electrophoresis gel.
- Size- and charge-matched dyes enable co-migration of labeled samples within the gel.
- Bright and highly sensitive dyes allow the use of the minimal labeling technique.
- Minimal loss of signal during labeling, separation, and scanning.
- No change in signal over wide pH range used during first-dimension (IEF) separation.
- Discrete signal from each fluor with minimal cross-talk contributes to high accuracy.
Componenti
Avvertenza
Stoccaggio e stabilità
Risultati analitici
Note legali
Cy3B: This product is manufactured under an exclusive license from Carnegie Mellon University and is covered by US patent number 6,133,445 and equivalent patents and patent applications in other countries.
CyQ (or Cy5Q or Cy7Q): These products are covered under US patent number 6,828,116 and equivalent patents and patent applications in other countries in the name of Cytiva.
CytoCy5S: The use of this product in an NTR gene reporter assay is the subject of US patent number 7,579,140 in the name of Cytiva.
CypHer5E: This product or portions thereof is manufactured under license from Carnegie Mellon University and is covered by US patent number 7,615,646.
Cy and CyDye, are - trademarks of Cytiva or one of its subsidiaries.
The purchase of CyDye products includes a limited license to use the CyDye products for internal research and development but not for any commercial purposes. A license to use the Cy and CyDye trademarks for commercial purposes is subject to a separate license agreement with Cytiva. Commercial use shall include:
1. Sale, lease, license or other transfer of the material or any material derived or produced from it.
2. Sale, lease, license or other grant of rights to use this material or any material derived or produced from it.
3. Use of this material to perform services for a fee for third parties, including contract research and drug screening.
If you require a commercial license to use the Cy and CyDye trademarks please contact LSlicensing@cytiva.com.
If you require a commercial license to use this material and do not have one, return this material unopened to Cytiva Bio-Sciences AB, Bjorkgatan 30, SE-751 84 Uppsala, Sweden and any money paid for the material will be refunded.
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Codice della classe di stoccaggio
13 - Non Combustible Solids
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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Articoli
Troubleshooting table for 2D DIGE results lists problems, causes, and prevention methods for future experiments.
Protocolli
This page describes two-dimensional separation of protein samples with Cytiva products.
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