GE17-6001-14
Immobiline® DryStrip pH 3-10
Cytiva 17-6001-14, pack of 13 cm
About This Item
Prodotti consigliati
Forma fisica
solid
Caratteristiche
wetted part: no
Confezionamento
pack of 13 cm
Produttore/marchio commerciale
Cytiva 17-6001-14
Colore
Colorless
Solubilità
cold water: soluble (Insoluble in cold and hot water)
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
Descrizione generale
Immobiline® DryStrip gels are available in fourteen overlapping pH ranges and five strip lengths (7, 11, 13, 18, and 24 cm) and represent the most comprehensive selection of narrow-, medium-, and wide-range IPG strips available. The five narrow pH range (1 pH unit), 24-cm IPG strips deliver the highest resolution and protein loading capacity available, which results in well separated spots with high protein content after the second-dimension separation.
The pH 3–11 NL strip covers most proteins found in prokaryotic and eukaryotic cells while the narrow-range strips can be used when higher resolution is required. The pH gradients at the extreme ends of the pH scale are nonlinear to distribute the proteins evenly over the gel length to obtain maximal resolution.
Immobiline® DryStrip gels are rehydrated with a matching IPG Buffer prior to use. Samples can be included directly in the rehydration solution for efficient loading of dilute samples or for loading larger amounts of protein. Samples can also be loaded after rehydration. The first-dimension separation is carried out using Ettan™ IPGphor 3 IEF system or Multiphor II flatbed system.
Applicazioni
Caratteristiche e vantaggi
- Maximize resolution, loading capacity, and reproducibility of first-dimension isoelectric focusing (IEF).
- Choose from a comprehensive range of overlapping Immobiline® DryStrip gels that cover narrow (1 pH unit), medium (3–5 pH units), and broad (8 pH units) pH ranges.
- Select the broad pH 3–11 NL gradient Immobiline® DryStrip gel for fast and efficient screening to gain a broad overview of total protein distribution.
- For more detailed studies or when using greater protein loads, choose from four new overlapping strips covering the pH 3 to 11 range.
- Higher resolution can be obtained using narrow-range (1 pH unit) Immobiline® DryStrip gels.
- Use matching IPG buffers for optimized running conditions.
- Use IPG strips directly after reswelling without further manipulation.
- Barcoded strips for convenient sample and batch tracking.
Stoccaggio e stabilità
Risultati analitici
Note legali
Codice della classe di stoccaggio
13 - Non Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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Protocolli
2-D Clean-Up Kit from Cytiva is designed to prepare samples that would otherwise produce poor 2-D results.
This page describes how to apply equilibrated Immobiline® DryStrip gels on the ExcelGel SDS gels.
This protocol shows how to prepare the Immobiline® DryStrip Kit and electrodes strips.
This page shows how to apply higher sample volumes and protein amounts with paper bridges in isoelectric focusing with Cytiva products
Contenuto correlato
This page shows how to apply equilibrated Immobiline DryStrip gels
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