GE17-5130-02

GSTrap^™ Fast Flow

Cytiva 17-5130-02, pack of 2 × 1 mL

• Codice UNSPSC: 23151817
• NACRES: NA.25

Proprietà

• ligand: glutathione and 10-carbon linker arm
• Confezionamento: pack of 2 × 1 mL
• Produttore/marchio commerciale: Cytiva 17-5130-02
• Parametri: <4 mL/min flow rate; 42 psi
• Dimensioni del letto: 7 mm × 25 mm
• Volume del letto: 1 mL
• Diam. int. colonna: 7 mm
• Matrice: highly cross-linked 4% agarose
• Diametro medio: 90 μm
• cleaning: 3-12
• Intervallo di esercizio: 3-12
• Capacità: >10 mg/mL binding capacity

Descrizione

Descrizione generale

GSTrap^™ FF columns are prepacked Glutathione Sepharose^™ Fast FLow columns for fast, convenient, one-step purification of glutathione S-transferase (GST) tagged proteins.

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Applicazioni

GST*-tagged Proteins can be purified directly from pretreated bacterial lysates using GSTrapTM FF.GST-tagged Proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves Protein antigenicity and function. If desired, cleavage of the Protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged Proteins can be detected using colorimetric or immunological methods. The medium, Glutathione SepharoseTM 4 Fast Flow, is also available as lab packages and is an excellent choice for scale-up.The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTATM design or FPLCTM System.

Caratteristiche e vantaggi

• Fast and simple one-step purification of GST-tagged Proteins.
• Mild elution conditions preserving Protein antigenicity and function.
• Easy scale-up by connecting the columns in series.
• Sepharose^™ 4 Fast FLow provides good fLow properties.

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Altre note

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Note legali

GSTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

Informazioni sulla sicurezza

• Pittogrammi: GHS02
• Avvertenze: Warning
• Indicazioni di pericolo: H226
• Consigli di prudenza: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Codice della classe di stoccaggio: 3 - Flammable liquids