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GE17-1128-01

MAbtrap® Kit

Cytiva 17-1128-01, pkg of 1 kit

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About This Item

Codice UNSPSC:
13111000
NACRES:
NA.56

ligand

recombinant protein G lacking albumin-binding region

Confezionamento

pkg of 1 kit

Produttore/marchio commerciale

Cytiva 17-1128-01

Condizioni di stoccaggio

(20% Ethanol)

Parametri

<4 mL/min flow rate
42 psi

Dimensioni del letto

7 mm × 25 mm

Volume del letto

1 mL

Diam. int. colonna

7 mm

Matrice

highly cross-linked 6% agarose

Dimens. media delle particelle

34 μm

cleaning

2-9

working range

3-9

Capacità

>1 mg binding capacity (lgG/column)

Temperatura di conservazione

2-8°C

Descrizione generale

MAbTrap® is an affinity chromatography kit, with everything required for small-scale IgG purification from ascites fluid, serum, and cell culture supernatants.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Applicazioni

MAbTrap® Kit is an affinity chromatography kit for fast and effective purification of monoclonal and polyclonal IgG from ascites fluid, serum, and cell culture supernatants. MAbTrap® Kit contains everything necessary to perform a complete one step purification without using complicated equipment. It also eliminates time-consuming buffer preparation and column packing. The kit includes one HiTrap® column, prepacked with Protein G Sepharose High Performance.

Caratteristiche e vantaggi

  • Convenient kit for purification of IgG from monoclonal and polyclonal material using a syringe in less than 15 min.
  • Kit contains one HiTrap® Protein G HP column prepacked with Protein G Sepharose High Performance and enough buffer concentrate for 20 preparations with a syringe.
  • MAbTrap® Kit contains everything necessary to perform a complete one-step purification without using complex equipment.

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Altre note

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Note legali

HiTrap is a registered trademark of Cytiva
MAbTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

Pittogrammi

Exclamation mark

Avvertenze

Warning

Indicazioni di pericolo

Codice della classe di stoccaggio

3 - Flammable liquids


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Articoli

Purify monoclonal or polyclonal IgG from serum, cell culture supernatant or ascitic fluid using the HiTrap Protein G HP from Cytiva, an affinity-exclusion chromatography product containing Sepharose-immobilized Protein G.

This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from Cytiva.

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protocolli

This page shows how to prepare samples for purification with affinity chromatography.

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