F9424

Fibroblast Growth Factor Receptor-2α (IIIb)/Fc Chimera human

>90% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder

• Sinonimo/i: FGFR-2α (IIIb)/Fc
• Numero MDL: MFCD03456786
• Codice UNSPSC: 51111800

Proprietà

• Origine biologica: human
• Ricombinante: expressed in baculovirus infected Sf21 cells
• Saggio: >90% (SDS-PAGE)
• Stato: lyophilized powder
• Potenza: 1-3 ng/mL ED₅₀
• PM: monomer calculated mol wt 66 kDa; ~100 kDa by SDS-PAGE (reducing)
• Condizioni di stoccaggio: avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)
• Impurezze: <1 EU/μg Endotoxin level (LAL test)
• N° accesso UniProt: P21802
• Temperatura di conservazione: −20°C
• Informazioni sul gene: human ... FGFR2(2263)

Descrizione

Descrizione generale

FGFR2 (fibroblast growth factor receptor 2) is a member of the tyrosine kinase receptor family called FGFR. This gene is localized to human chromosome 10q26, and is composed of 2 exons.
Its ligand-binding domain in present in its extracellular region, which is composed of three immunoglobulin (Ig)-like domains. It spans the membrane once, and its tyrosine kinase domain is localized to its intracellular region. Alternative splicing of this gene gives rise to two isoforms- epithelial (FGFR2IIIb) and mesenchymal (FGFR2IIIc).

Azioni biochim/fisiol

FGFR2 (fibroblast growth factor receptor 2) facilitates tumor growth, invasiveness, metastasis and angiogenesis. It plays an essential role in the development of mammary glands, and its expression is elevated in breast cancers. Studies in North Indian population suggest that polymorphisms in this gene might be linked to susceptibility to breast cancer. It is anti-apoptotic in nature, and suppresses p53-mediated apoptosis, by inducing the elevation of MDM2 levels. Mutations in this gene are linked to mild Crouzon syndrome and craniosynostosis.

Stato fisico

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline

Risultati analitici

The bioactivity is measured by its ability to inhibit human fibroblast growth factor acidic-dependent proliferation of NR6 cells.

Altre note

Human recombinant FGFR-2α (IIIb)/Fc is a disulfide-linked homodimeric protein. As a result of glycosylation, it migrates as an approximately 100 kDa protein.