F9015

Fluorescamina

≥98% (TLC), powder, used for detection of primary amines

• Sinonimo/i: 4-fenilspiro-[furan-2(3H),1-ftalan]-3,3′-dione
• Formula empirica (notazione di Hill): C₁₇H₁₀O₄
• Numero CAS: 38183-12-9
• Peso molecolare: 278.26
• UNSPSC Code: 12352204
• NACRES: NA.32
• PubChem Substance ID: 24894984
• EC Number: 253-814-5
• Beilstein/REAXYS Number: 921143
• MDL number: MFCD00005928

Proprietà

• assay: ≥98% (TLC)
• form: powder
• color: off-white to yellow
• mp: 153-157 °C (lit.)
• solubility: acetone: 50 mg/mL
• storage temp.: room temp
• SMILES string: O=C1OC2(OC=C(C2=O)c3ccccc3)c4ccccc14
• InChI: 1S/C17H10O4/c18-15-13(11-6-2-1-3-7-11)10-20-17(15)14-9-5-4-8-12(14)16(19)21-17/h1-10H
• InChI key: ZFKJVJIDPQDDFY-UHFFFAOYSA-N

Descrizione

General description

Fluorescamine, a heterocyclic dione, reacts with primary amines, amino acids, peptides, and protein to form a fluorescent product. Excess reagent and its degradation products are non-fluorescent. The fluorescence of a solution containing protein plus fluorescamine is equivalent to the quantity of free amine groups present. This is the basic principle of a fluorescent protein assay. Fluorescamine is used as a derivatizing agent for the identification of various compounds used in the pharmaceutical industry, such as oseltamavir, penicillamine, sulfonamide residues, lisinopril, methotrexate, vigabatrin, and gabapentin.

Application

Reagente non fluorescente che reagisce rapidamente in condizioni lievi con ammine primarie in aminoacidi e peptidi per formare composti stabili, altamente fluorescenti. Basso background dovuto all′idrolisi. Utile nel saggio fluorometrico di aminoacidi, proteine ed enzimi proteolitici. È efficace nel bloccare gruppi amino terminali generati di recente nelle analisi delle sequenze proteiche.

Fluorescamine has been used as a stain to localize granular proteins on the surface of foxtail millet starch as well as protein in foxtail millet flour through confocal scanning laser microscopy (CSLM). It has also been used for photometric determination of para-aminobenzoic acid.

Informazioni sulla sicurezza

• Classe di stoccaggio: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)