F3665

Lipopolisaccaridi

FITC conjugate

• Sinonimo/i: LPS
• Numero CAS: 93572-42-0
• UNSPSC Code: 12352201
• EC Number: 297-473-0
• NACRES: NA.25
• MDL number: MFCD00164401

Proprietà

• biological source: Escherichia coli (O111:B4)
• Quality Segment: 200
• conjugate: FITC conjugate
• form: lyophilized powder
• extent of labeling: 2-10 μg FITC per mg LPS
• color: yellow to orange
• solubility: H₂O: soluble 4.90-5.10 mg/mL, faintly hazy to hazy, pale yellow to yellow
• storage temp.: 2-8°C

Descrizione

Application

FITC-Lipopolysaccharides (FITC-LPS) may be used to study processes such as the movement/transport of LPS across tissue barriers such as the colonic epithelium and cellular binding and internalization of lipopolysaccharides with fluorescence-based assay systems. It may be used to evaluate, qualify and quantify lipopolysaccharides (LPS) removal reagents and procedures. FITC-LPS conjugate may be used for the detection of LPA binding proteins and motifs.

I lipopolisaccaridi (LPS) sono componenti caratteristici della parete cellulare dei batteri gram negativi. Il LPS e la sua frazione lipide A stimolano le cellule del sistema immunitario innato attraverso il recettore Toll-like 4 (TLR4), un elemento della famiglia delle proteine recettoriali Toll-like, che riconosce i profili molecolari associati ai patogeni (PAMP) comuni.

Biochem/physiol Actions

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Preparation Note

The product is soluble in water (5 mg/ml) yielding a hazy, yellow to orange solution. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution. For cell culture use, LPS should be reconstituted by adding 1 ml of sterile balanced salt solution or cell culture medium to a vial (1 mg) and swirling gently until the powder dissolves. Solutions can be further diluted to the desired working concentration with additional sterile balanced salt solutions or cell culture media.

Other Notes

To gain a comprehensive understanding of our extensive range of lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Informazioni sulla sicurezza

• Pittogrammi: GHS06
• Parola di avvertenza: Danger
• Codici di pericolo: H300
• Avvertenze di sicurezza: P264 - P270 - P301 + P310 - P405 - P501
• Hazard Classifications: Acute Tox. 2 Oral
• Classe di stoccaggio: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Che succede?: WGK 3
• Punto di infiammabilità (°F): Not applicable
• Punto di infiammabilità (°C): Not applicable
• DPI (dispositivi di protezione individuale): Eyeshields, Gloves, type N95 (US)