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E8027

Sigma-Aldrich

EphA2/Fc Chimera from mouse

>95% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder

Sinonimo/i:

Eck, Myk2, Sek

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About This Item

Numero MDL:
Codice UNSPSC:
51111800

Origine biologica

mouse

Ricombinante

expressed in NSO cells

Saggio

>95% (SDS-PAGE)

Stato

lyophilized powder

Potenza

0.078-5 ng/mL

PM

monomer calculated mol wt 84 kDa
~105 kDa by SDS-PAGE (reducing)

Confezionamento

pkg of 200 μg

Condizioni di stoccaggio

avoid repeated freeze/thaw cycles

tecniche

ligand binding assay: suitable

Impurezze

endotoxin, tested

N° accesso UniProt

Temperatura di conservazione

−20°C

Informazioni sul gene

mouse ... Epha2(13836)

Descrizione generale

EPHA2 (ephrin receptor A2) belongs to the Eph family of receptor tyrosine kinases. It is a type I transmembrane protein, and its exoplasmic domain consists of a ligand binding domain in its N-terminal, a cysteine-rich domain, and two fibronectin type III repeats. Its cytoplasmic domain contains a juxtamembrane region, a tyrosine kinase domain, a SAM (sterile-a-motif) motif, and a PDZ-binding domain.

Azioni biochim/fisiol

EPHA2 binds to the ephrin-A ligand and has diverse cellular function. EPHA2 has been shown to be an oncoprotein of importance in a range of cancers. EPHA2 is overexpressed in several human cancer types and promotes malignancy through a mechanism involving RhoA-dependent destabilization of adherens junctions. EPHA2 overexpression induces a FAK-dependent increase in MMP-2 expression and invasiveness and this process that can be reversed by ligation of EPHA2.
Member of the Eph receptor tyrosine kinase family shown to bind ephrin-A1, ephrin-A2, ephrin-A3, ephrin-A4, and ephrin-A5; involved in pattern formation and morphogenesis. EphA2 is expressed on endothelial cells and in tumor xenographs and may regulate tumor neovascularization.

Altre note

Extracellular domain of mouse EphA2 (amino acids 1-535) fused by means of a polypeptide linker to the Fc region of human IgG1 that is histidine-tagged at the C-terminus.

Stato fisico

Lyophilized from a 0.2 μm filtered solution in 20 mM Tris, pH 8.0.

Risultati analitici

The biological activity is measured by its ability to bind recombinant mouse ephrin-A1/Fc in an ELISA assay.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


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Mark S Duxbury et al.
Biochemical and biophysical research communications, 320(4), 1096-1102 (2004-07-14)
The Eph tyrosine kinases interact with ligands of the Ephrin family and have diverse cellular functions. EphA2 has been recognized to be an oncoprotein of importance in a range of cancers. Here, we examine the effect of EphA2 overexpression and
Wei Bin Fang et al.
Journal of cell science, 121(Pt 3), 358-368 (2008-01-17)
EPHA2 receptor tyrosine kinase is overexpressed in several human cancer types and promotes malignancy. However, the mechanisms by which EPHA2 promotes tumor progression are not completely understood. Here we report that overexpression of a wild-type EPHA2, but not a signaling-defective
Linda M Reis et al.
Molecular vision, 20, 836-842 (2014-06-19)
Congenital cataracts occur in 3-4 per 10,000 live births and account for 5% to 20% of pediatric blindness worldwide. With more than 37 genes known to be associated with isolated congenital cataract, whole exome sequencing (WES) was recently introduced as

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