E6157

Anti-eIF2Bβ (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Sinonimo/i: Anti-EIF-2B, Anti-EIF2B-beta, Anti-EIF2BB, Anti-Eukaryotic translation initiation factor 2B, beta, Anti-Eukaryotic translation initiation factor 2B, subunit 2
• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Coniugato: unconjugated
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Stato: buffered aqueous solution
• PM: antigen ~39 kDa
• Reattività contro le specie: rat, mouse, human
• Concentrazione: ~1.0 mg/mL
• tecniche: immunoprecipitation (IP): 5-10 μg using K562 cell lysates; western blot: 1-2 μg/mL using K562 cell lysates
• N° accesso UniProt: P49770
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... EIF2B2(8892) ; mouse ... Eif2b2(217715) ; rat ... Eif2b2(84005)

Descrizione

Descrizione generale

Translation initiation factor eIF-2B subunit beta, eIF2B β is a component of the regulatory subcomplex of eIF2B along with the eIF2Bα and -δ subunits. eIF2Bβ gene is mapped to human chromosome 14q24.3.

Specificità

Anti-eIF2Bβ (C-terminal) specifically recognizes human, mouse, and rat eIF2Bβ.

Applicazioni

Anti-eIF2Bβ (C-terminal) antibody produced in rabbit is suitable for immunoprecipitation at a concentration of 5-10μg using K562 cell lysates and western blotting at a concentration of 1-2μg/mL using K562 cell lysates.

Anti-eIF2Bβ (C-terminal) antibody produced in rabbit may be used in immunoblotting.

Azioni biochim/fisiol

The eIF2Bα,-β, and -δ subunits downregulate eIF2B activity in response to the phosphorylation of eIF2 on Ser51. Recent studies have linked inherited mutations in any of the five eIF2B subunits to a fatal human disorder known as childhood ataxia with central nervous system hypomyelination (CACH) or vanishing white matter (VWN) disease.

Translation initiation factor eIF-2B subunit β is a part of a five-subunit complex that catalyzes guanine nucleotide exchange on eIF2. EIF2B2 is associated with the cause of autosomal recessive disorders. Autosomal recessive inherited mutations in each of the five eukaryotic initiation factor 2B (eIF2B) subunits are known to cause white matter abnormalities called eIF2B-related disorders.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stoccaggio e stabilità

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)