The molecular weight for this product is not determined. However, other sources report a molecular weight of 24,000 daltons for Diaphorase from Clostridium.
D2197
Diaphorase from Clostridium kluyveri
recombinant, expressed in E. coli, Animal-component free
Sinonimo/i:
Diaphorase, Lipoamide Dehydrogenase, Lipoyl Dehydrogenase
Autenticati per visualizzare i prezzi organizzativi e contrattuali.
Scegli un formato
100 UNITS
CHF 82.80
300 UNITS
CHF 213.00
500 UNITS
CHF 276.00
Vai a
Servizio Tecnico
Hai bisogno di aiuto? Il nostro team di scienziati qualificati è a tua disposizione.
Permettici di aiutartirecombinant
expressed in E. coli
Quality Level
form
solid
specific activity
>=30 units/mg protein (biuret)
storage temp.
−20°C
1 of 4
Questo articolo | |||
|---|---|---|---|
| specific activity >=30 units/mg protein (biuret) | specific activity 3.0-20.0 units/mg protein (biuret) | specific activity ≥400 units/mg protein (biuret) | specific activity ≥6 U/mg protein |
| recombinant expressed in E. coli | recombinant - | recombinant - | recombinant - |
| form solid | form lyophilized powder | form ammonium sulfate suspension | form ammonium sulfate suspension |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level - | Quality Level 100 |
General description
Research area: Cancer
Application
Diaphorase from Clostridium kluyveri has been used:
- in lactate dehydrogenase assay in gill cells.
- impregnated on the strip for formate dehydrogenase assay.[1]
- in various assays to oxidate β-NADH or β-NADPH in the presence of an electron acceptor.
- for semiquinone generation during benzoquinone reduction.[2]
- for evaluation of glucose uptake in cultured muscle cells.[3]
- in lactate dehydrogenase cytotoxicity assay.
Biochem/physiol Actions
Diaphorase catalyzes the oxidation of either β-NADH or β-NADPH in the presence of an electron acceptor such as methylene blue or 2,6-dichlorophenolindophenol. Diaphorases that are specific for either β-NADH or β-NADPH are known. The pig heart enzyme of Straub seems to have native diaphorase (β-NADH specific) as well as lipoic and lipoamide dehydrogenase activities.
Physical form
Supplied as a lyophilized powder containing trehalose.
Other Notes
One unit of diaphorase will oxidize 1.0 micromole of beta-NADH per minute at pH 7.5 at 25 degrees C, with the corresponding reduction of the appropriate electron acceptor.
Classe di stoccaggio
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Scegli una delle versioni più recenti:
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Transient Agarose Spot (TAS) Assay: A New Method to Investigate Cell Migration
Veres-Szekely A, et al.
International Journal of Molecular Sciences, 23(4), 2119-2119 (2022)
Relationship between insulin sensitivity and gene expression in human skeletal muscle
Parikh HM, et al.
BMC endocrine disorders, 21, 32-32 (2021)
Apor Veres-Székely et al.
International journal of molecular sciences, 23(4) (2022-02-27)
Fibroblasts play a central role in diseases associated with excessive deposition of extracellular matrix (ECM), including idiopathic pulmonary fibrosis. Investigation of different properties of fibroblasts, such as migration, proliferation, and collagen-rich ECM production is unavoidable both in basic research and
Knut Erik Hovda et al.
Scandinavian journal of clinical and laboratory investigation, 75(7), 610-614 (2015-07-24)
The standard diagnostic approach to methanol poisoning is chromatographic measurement of methanol on centrally placed stationary equipment. Methanol poisoning in places where such equipment is unavailable is thus often not diagnosed. Methanol is metabolized to a toxic metabolite, formate; the
Claudio F Gonzalez et al.
The Journal of biological chemistry, 280(24), 22590-22595 (2005-04-21)
Most bacteria contain soluble quinone-reducing flavoenzymes. However, no biological benefit for this activity has previously been demonstrated. ChrR of Pseudomonas putida is one such enzyme that has also been characterized as a chromate reductase; yet we propose that it is
Numero articolo commerciale globale
| SKU | GTIN |
|---|---|
| D2197-300UN | 04061832729251 |
| D2197-500UN | 04061833561867 |
| D2197-100UN | 04061832729244 |
-
What is the molecular weight of this diaphorase?
1 answer-
Helpful?
-
-
How to prepare stock solution for Diaphorase in order to use 2 units in reaction
1 answer-
For assay purposes, this product is reconstituted 1 mg/ml in cold 200 mM Tris-HCl buffer containing 294 mM Potassium Chloride, 0.54 mM Riboflavin 5'-monophosphate, and 0.025% (w/v) BSA, pH 7.5. The minimum activity of this enzyme is 30 u/mg protein with a minimum protein content of 50%. At the minimum activity and minimum protein content, 2 units of 1 mg/mL stock solution would have a volume of 133 uL. Please see the link below to review a sample or lot-specific Certificate:
https://www.sigmaaldrich.com/product/sigma/d2197#product-documentationHelpful?
-
Active Filters
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica