The kit does not have specified lower and upper detection limits for enzyme activity. The enzyme activity needs to be empirically determined using varying enzyme amounts.
CS0780
β-N-Acetylglucosaminidase Assay Kit
sufficient for 50 reactions (1 mL), sufficient for 500 reactions (100 μL)
Sinonimo/i:
Beta-NAG Activity Assay Kit
Autenticati per visualizzare i prezzi organizzativi e contrattuali.
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1 KIT
CHF 604.00
Informazioni su questo articolo
UNSPSC Code:
12161503
EC Number:
222-398-7
NACRES:
NA.84
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Permettici di aiutartiusage
sufficient for 50 reactions (1 mL), sufficient for 500 reactions (100 μL)
shipped in
wet ice
storage temp.
2-8°C
Quality Level
Gene Information
human ... NAGLU(4669)
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Categorie correlate
1 of 4
Questo articolo | CS0720 | CS1090 | CS0170 |
|---|---|---|---|
| Gene Information human ... NAGLU(4669) | Gene Information human ... CS(1431) | Gene Information human ... HMGCR(3156) | Gene Information human ... TXNRD1(7296), TXNRD2(10587), TXNRD3(114112) |
| Quality Level 200 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
| usage sufficient for 50 reactions (1 mL), sufficient for 500 reactions (100 μL) | usage kit sufficient for 100 reactions (using a 1 ml cuvette), kit sufficient for 480 reactions (using 96 multiwell plates) | usage sufficient for 100 assays (200 μL), sufficient for 30 assays (1 mL) | usage kit sufficient for 100 assays (1 mL) |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −70°C | storage temp. −20°C |
| shipped in wet ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
Application
The kit provides the reagents required for a fast and convenient detection of β-N-Acetylglucosaminidase activity in cell lysates, tissue homogenates or purified enzyme. It is a useful tool for the detection of lysosomes in fractionated cell/tissue samples.
Biochem/physiol Actions
N-acetyl-D-glucosaminidase (NAG) in mammals is a lysosomal enzyme, which takes part in the intracellular degradation of glycolipids and glycoproteins. High activities of this enzyme have been detected in human kidney, lung and liver lysosomes. Elevated levels of serum NAG are associated with certain forms of cancer.
Analysis Note
The kit assay is based on the hydrolysis of NAG substrate (NP-GLcNAc) by the enzyme. The enzymatic hydrolysis of the substrate liberates p-nitrophenylate ion. The reaction product is detected colorimetrically at 405 nm.
Solo come componenti del kit
N° Catalogo
Descrizione
- Dilution Buffer 8 mL
- 4-Nitrophenyl-N-acetyl-β-D-glucosaminide 50 mg
- Citrate Buffer Solution, 0.09 M 100 mL
- p-Nitrophenol Standard Solution, 10 mM 1 mL
- β-N-Acetylglucosaminidase from Jack beans 1 vial
- Sodium carbonate 5 g
related product
N° Catalogo
Descrizione
Determinazione del prezzo
signalword
Warning
hcodes
Hazard Classifications
Carc. 2 - Eye Irrit. 2 - STOT RE 2 Oral
target_organs
Liver,Kidney
wgk
WGK 3
Classe di stoccaggio
10 - Combustible liquids
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What are the lower and upper limits of enzyme activity that the CS0780 B-N-Acetylglucosaminidase Assay Kit can measure?
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