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CS0030

Sigma-Aldrich

Senescence Cells Histochemical Staining Kit

sufficient for 100 tests

Sinonimo/i:

Senescent cells IHC kit, cellular β-galactosidase probe

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About This Item

Codice UNSPSC:
12352207
NACRES:
NA.32

impiego

sufficient for 100 tests

Livello qualitativo

200

Confezionamento

pkg of 1 kit

Condizioni di stoccaggio

dry at room temperature

Metodo di rivelazione

colorimetric

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

Descrizione generale

The Senescence Cells Histochemical Staining Kit contains all the reagents required for identifying senescent cells using an assay based on a histochemical stain for ß-galactosidase activity at pH 6. ß-galactosidase activity is detectable in senescent cells, but not in quiescent, immortal, or tumor cells.
The Senescence Cells Histochemical Staining Kit contains all the reagents required for identifying senescent cells using an assay based on a histochemical stain for β-galactosidase activity at pH 6. β-galactosidase activity is detectable in senescent cells, but not in quiescent, immortal, or tumor cells. Cellular senescence is a progression of events, whereby cells move from an actively dividing to a non-dividing stage. The cellular senescence process is associated with aging. The decrease in cell division is virtually irreversible and complete. In conjunction with the loss of the ability to divide, changes occur in the morphology, shape, and physical appearance of the cells, and their pattern of gene expression. At the end of the process cell death usually occurs, although the cells may remain viable for a long time.

Applicazioni

Senescence Cells Histochemical Staining Kit has been used for:
  • senescence-associated β-galactosidase assay
  • to test for senescence in human pancreatic cancer cells
  • adipose-derived stem cells (ADSCs)
  • cord blood mesenchymal stromal/stem cells (CBMSC)

Azioni biochim/fisiol

Replicative senescence is a growth-arrest state associated with loss of division potential, changes in cell morphology, shape and physical appearance, and the pattern of gene expression in cells.

Note legali

Manufactured under license to US Patent Nos. 5,491,069 and 5,795,728.

Solo come componenti del kit

N° Catalogo
Descrizione
  • X-gal solution 4 mL
  • Staining Solution, 10X 15 mL
  • Fixation Buffer 10x 15 mL
  • Reagent B 1.5 mL
  • Reagent C 1.5 mL
  • Dulbecco's Phosphate Buffered Saline (PBS) 10x 60 mL

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β-Galactosidase stain

Avvertenze

Danger

Classi di pericolo

Acute Tox. 3 Inhalation - Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Muta. 2 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3

Organi bersaglio

Respiratory system

Rischi supp

EUH032

Codice della classe di stoccaggio

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Certificati d'analisi (COA)

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Sigma-Aldrich

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B van der Loo et al.
Experimental cell research, 241(2), 309-315 (1998-06-25)
A beta-galactosidase activity has recently been used as a histochemical marker of replicative senescence in human fibroblasts and keratinocytes. To establish whether this marker could be used to detect senescence of vascular cells, we have investigated its presence in cultures
G P Dimri et al.
Proceedings of the National Academy of Sciences of the United States of America, 92(20), 9363-9367 (1995-09-26)
Normal somatic cells invariably enter a state of irreversibly arrested growth and altered function after a finite number of divisions. This process, termed replicative senescence, is thought to be a tumor-suppressive mechanism and an underlying cause of aging. There is
Mandy Oj Grootaert et al.
Autophagy, 11(11), 2014-2032 (2015-09-24)
Autophagy is triggered in vascular smooth muscle cells (VSMCs) of diseased arterial vessels. However, the role of VSMC autophagy in cardiovascular disease is poorly understood. Therefore, we investigated the effect of defective autophagy on VSMC survival and phenotype and its
Natalja E Fedorovich et al.
Biomaterials, 30(3), 344-353 (2008-10-22)
Photopolymerizable hydrogels, formed by UV-exposure of photosensitive polymers in the presence of photoinitiators, are widely used materials in tissue engineering research employed for cellular entrapment and patterning. During photopolymerization, the entrapped cells are directly exposed to polymer and photoinitiator molecules.
Phuc H Pham et al.
In vitro cellular & developmental biology. Animal, 53(1), 20-32 (2016-09-03)
As diseases and abnormalities of the heart can interfere with the aquaculture of Atlantic salmon, the heart was investigated as a source of cell lines that could be used to study the cellular basis of these conditions. An Atlantic salmon
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