CLL1097
A549 CELLS EGFR BIOSENSOR
human male lung (Source Disease: Lung carcinoma)
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| Voi/SKU | Disponibilità | Prezzo |
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1 vial | Per conoscere la disponibilità, visualizza il carrello | CHF 8900.00 |
Informazioni su questo articolo
UNSPSC Code:
41106514
NACRES:
NA.81
Biological source:
human male lung (Source Disease: Lung Carcinoma)
Servizio Tecnico
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Permettici di aiutartiNome del prodotto
A549 CELLS EGFR BIOSENSOR,
biological source
human male lung (Source Disease: Lung Carcinoma)
Quality Segment
OMIM accession no.
storage temp.
−196°C
Gene Information
human ... EGFR(1956), ERBB1(1956)
General description
This cell line was derived from ATCC catalog No. CCL-185.
Application
The EGFR Biosensor cell line consists of the SH2 domain of the adaptor protein Grb2 fused to GFP. The biosensor was transduced into the A549 cell line and a stable cell line was cloned from a single cell. Within minutes after stimulation of the cell line with EGF, the biosensor shows robust redistribution towards the cell membrane and subsequent internalization through endocytosis. The internalization kinetics of the sensor-receptor complex can be quantified by image analysis software to count granules. The reporter response is highly selective as it can be induced only by specific EGFR ligands and is abolished by a selective inhibitor of EGFR, Tyrphostin AG 1478.
To learn more, please visit the EGFR Biosensor Cell Line webpage
Features and Benefits
Lentiviral transduction of DNA construct for stable expression of GFP-tagged SH2 binding domain.
These A549 cells are adherent with a doubling time of approx. 22 hours.
Preparation Note
Cell freezing medium-DMSO 1X, Cat. No. C6164
Media renewal changes two to three times per week.
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C.
The base medium for this cell line is RPMI-1640, Cat. No. R0883. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F2442, to a final concentration (v/v) of 10%, L-glutamine, Cat. No. G7513, at a final concentration of 2 mM and puromycin, Cat. No. P9620, at a final concentration of 1 μg/mL.
Analysis Note
Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation.
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Questo articolo | |||
|---|---|---|---|
| description - | description - | description - | description - |
| biological source human male lung (Source Disease: Lung Carcinoma) | biological source human male lung (Source Disease: Lung carcinoma) | biological source human male lung (Source Disease: Lung carcinoma) | biological source human male lung (Source Disease: Lung carcinoma) |
| storage temp. −196°C | storage temp. −196°C | storage temp. −196°C | storage temp. −196°C |
| Gene Information human ... EGFR(1956), ERBB1(1956) | Gene Information human ... EGFR(1956), ERBB1(1956) | Gene Information human ... STAT3(6774) | Gene Information human ... STAT1(6772) |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| OMIM accession no. | OMIM accession no. | OMIM accession no. | OMIM accession no. |
Classe di stoccaggio
6.2 - Infectious substances
Che succede?
WGK 3
Punto di infiammabilità (°F)
Not applicable
Punto di infiammabilità (°C)
Not applicable
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