CLL1035
U2OS RFP-ACTB
human female bone (Disease source: Osteosarcoma)
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| Voi/SKU | Disponibilità | Prezzo |
|---|---|---|
1 vial | Per conoscere la disponibilità, visualizza il carrello | CHF 4960.00 |
Informazioni su questo articolo
UNSPSC Code:
41106514
NACRES:
NA.81
Biological source:
human female bone (Source Disease: Osteosarcoma)
Servizio Tecnico
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Permettici di aiutartiNome del prodotto
U2OS RFP-ACTB,
biological source
human female bone (Source Disease: Osteosarcoma)
Quality Segment
OMIM accession no.
storage temp.
−196°C
Gene Information
human ... beta actin(60)
General description
U2OS RFP-ACTB are osteosarcoma, epithelial cells, from a human caucasian female (aged 15 years), with a RFP-tagged modification.
Application
This product is a human U2OS cell line in which the genomic ACTB gene has been endogenously tagged with a Red Fluorescent Protein (RFP) gene using CompoZr® Zinc Finger Nuclease technology. Integration resulted in endogenous expression of the fusion protein in which RFP is attached to the N-terminus of actin. Fluorescence imaging shows that the actin can polymerize to form the characteristic pattern of actin filaments. Addition of Cytochalasin B to the cell line causes the expected shortening of actin filaments. This stable cell line was expanded from a single clone. The target′s gene regulation and corresponding protein function are preserved in contrast to cell lines with overexpression via an exogenous promoter.
To learn more, please visit the Cellular Reporter Cell Line webpage
Features and Benefits
RFP-tagged on chromosome 7p22.1
The U2OS cells are adherent, with a doubling time of approx. 29 hours.
Preparation Note
Media Renewal changes two to three times per week.
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.
Subculture Ratio: approx. 1:3-1:6
The base medium for this cell line is McCoy′s 5A1 Medium Modified, Cat. No. M9309. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration of 10%.
The cell freezing medium is DMSO 1X (Cat. No. C6164).
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.
Subculture Ratio: approx. 1:3-1:6
The base medium for this cell line is McCoy′s 5A1 Medium Modified, Cat. No. M9309. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration of 10%.
The cell freezing medium is DMSO 1X (Cat. No. C6164).
Analysis Note
Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.
Other Notes
U2OS RFP-ACTB cells
Legal Information
CompoZr is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
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Questo articolo | |||
|---|---|---|---|
| description - | description - | description - | description - |
| biological source human female bone (Source Disease: Osteosarcoma) | biological source human female bone (Source Disease: Osteosarcoma) | biological source human female bone (Source Disease: Osteosarcoma) | biological source human female bone (Disease source: Osteosarcoma) |
| storage temp. −196°C | storage temp. −196°C | storage temp. −196°C | storage temp. −196°C |
| Gene Information human ... beta actin(60) | Gene Information human ... beta actin(60) | Gene Information human ... ACTB(60), TUBA1B(10376) | Gene Information human ... ACTB(60), LMNB1(4001) |
| OMIM accession no. | OMIM accession no. | OMIM accession no. | OMIM accession no. |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Classe di stoccaggio
10 - Combustible liquids
Che succede?
WGK 3
Punto di infiammabilità (°F)
188.6 °F - closed cup
Punto di infiammabilità (°C)
87 °C - closed cup
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