PEA-10

97040310, mouse unknown/unspecified, Fibroblast

• Codice UNSPSC: 41106514

Proprietà

• product name: PEA-10, 97040310
• Origine biologica: mouse unknown/unspecified
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Fibroblast
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Mouse embryo fibroblast, LRP-deficient, heterozygous

Descrizione della linea cellulare

The cell line PEA-10 is heterozygous for a disruption of the gene for low density lipoprotein receptor-related protein (LRP). PEA-10 was derived from fibroblasts from day 15 C57BL/6x129 F1 hybrid mouse embryos. Together with the cell lines PEA-13 (Sigma Catalogue number. 97040311) and MEF-1 (Sigma Catalogue number. 98061101) an experimental system is available for the analysis of cellular uptake of functionally diverse ligands and the effect of LRP deficiency. This is of importance since LRP deficient mouse embryos die early during gestation.

Applicazioni

Study of LRP and LRP deficiency.

Terreno di coltura

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split confleunt cultures 1:6 to 1:10 i.e. seeding 2-4 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C

Altre note

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