EMT6/VCR/R

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: mouse unknown/unspecified
• Confezionamento: tube of 5 μg 96042323-DNA-5UG; pkg of vial of cells 96042323-1VL
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Epithelial-like
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Mouse mammary, drug-resistant

Descrizione della linea cellulare

EMT6/VCR/R is a drug-resistant variant of the mouse mammary tumour cell line EMT6/P (Sigma Catalogue number. 96042344). It was derived from the parent line by continuous growth in increasing concentrations of vincristine. EMT6/VCR/R was reported to be cross-resistant to other drugs of the multidrug-resistance (MDR) phenotype, showed reduced intracellular drug accumulation and hyperexpression of P-glycoprotein.

Applicazioni

Drug-resistance studies

Terreno di coltura

MEM(EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1μg/ml vincristine + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37ºC. It is recommended to resusitate cells in drug-free medium. Add drug after growth is established.

Altre note

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