Psi-CRIP

94090901, mouse embryo, Epithelial

• Codice UNSPSC: 41106514

Proprietà

• product name: Psi-CRIP, 94090901
• Origine biologica: mouse embryo
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Epithelial
• Prodotti: Amphotrophic helper-free recombinant retroviral packaging cell line.
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Mouse NIH 3T3 transformed with Moloney murine leukaemia mutants

Descrizione della linea cellulare

From a set of packaging cell lines useful for generating helper-free recombinant retroviruses with amphotropic host ranges. Two mutant Moloney murine leukaemia virus-derived proviral genomes carrying complementary mutations in the gag-pol or env regions were sequentially introduced into NIH 3T3 cells by co-transformation. Each genome contained a deletion of the Psi sequence necessary for the efficient encapsulation of retroviral genomes into virus particles and additional alterations at the 3′end of the provirus. Psi-CRIP can be used to isolate clones that stably produce high titres (1000,000cfu/ml) of recombinant retroviruses with amphotropic host ranges. Viral producers derived from Psi-CRIP do not transfer the packaging function or yield helper virus.

Applicazioni

Amphotropic helper-free recombinant retroviral packaging

Terreno di coltura

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (NB. The results obtained by the depositor were gained using 10% Calf Serum (CS)).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5-10% CO₂, 37°C.

Altre note

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.