KB (HeLa derivative)

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 94050408, human cervix, Epithelial-like

• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: human cervix
• Confezionamento: tube of 5 μg 94050408-DNA-5UG; pkg of vial of cells 94050408-1VL
• Modalità di accrescimento: Adherent
• Cariotipo: 2n = 46
• Morfologia: Epithelial-like
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human cervix carcinoma

Descrizione della linea cellulare

Originally derived from an epidermoid carcinoma of the mouth of an adult male Caucasian. It was one of the early attempts to isolate and serially propagate a human cell line directly on glass as a monolayer. NB: This cell line contains HeLa marker chromosomes and expresses type A G6PD. For further information see Nature 1976;259:211; In Vitro 1978;14:469. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Ethnicity: Black.

Applicazioni

KB has been used extensively in studies of cell nutrition and metabolism, cancer chemotherapy screening, tumourigenicity and viruses e.g. susceptible to Polio virus type I and Adenovirus type 3.

Terreno di coltura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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