Established from a biopsy of a radiosensitive patient. The patient was diagnosed as not having ataxia-telangiectasia, an autosomal recessive disorder where sensitivity to ionising radiation has been documented.
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
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