CCRF-SB

89090405, human blood, Lymphoblastoid

• Codice UNSPSC: 41106514

Proprietà

• product name: CCRF-SB, 89090405
• Origine biologica: human blood
• Modalità di accrescimento: Suspension
• Cariotipo: 2n = 46
• Morfologia: Lymphoblastoid
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human Caucasian acute lymphoblastic leukaemia

Descrizione della linea cellulare

A B lymphoblastoid line obtained from the peripheral blood of an 11 year old boy with acute lymphoblastic leukaemia. Appear free of virus particles by electron microscopy. Do not synthesise immunoglobulins. Grown in continuous spinner culture for 6 months. HLA profile: A1, A2, B12, B17, Cw2.

Applicazioni

HLA profile studies

Terreno di coltura

RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Maintain cultures between 3x10,000 to 1x100,000 cells/ml; 5% CO₂; 37°C. Cultures can tolerate high densities i.e. 1-2x1,000,000 cells/ml.

Altre note

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