RAG

89040605, mouse kidney, Ameboid-like

• Codice UNSPSC: 41106514

Proprietà

• product name: RAG, 89040605
• Origine biologica: mouse kidney
• Modalità di accrescimento: Adherent
• Cariotipo: Modal no. 68, hypertriploid
• Morfologia: Ameboid-like
• Prodotti: ES-2
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Mouse BALB/c renal adenocarcinoma

Descrizione della linea cellulare

Derived from the original Renal-2a BALB/c renal adenocarcinoma. Cells produce the unique kidney specific esterase ES-2 and have been used in somatic cell hybridisation studies because of their azaguanine resistant properties.

Applicazioni

Hybridisation, resistance and tumourigenicity studies

Terreno di coltura

EMEM (HBSS) + 2mM Glutamine + 2% Non Essential Amino Acids (NEAA) + 2% Vitamins + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Altre note

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.