TR33B

85040102, rat brain, Glial

• Codice UNSPSC: 41106514

Proprietà

• product name: TR33B, 85040102
• Origine biologica: rat brain
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Glial
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Rat Wistar-Furth oligodendroglioma

Descrizione della linea cellulare

Ethyl nitrosourea induced tumour of the spinal cord and roots from a Wistar-Furth rat. In cell culture the cells grow to a high density and form a sheet of cells with many processes. TR33B is the parent line of 33B (Sigma Catalogue number. 85081901).

Applicazioni

Tumourigenicity studies

Terreno di coltura

DMEM + 2mM Glutamine + 10% Tryptose Phosphate Broth + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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