AB-1 HA

12070506, mouse mesothelium, epithelial; small, spindly morphology, cobblestone at confluence

• Sinonimo/i: AB 1 HA, AB-1HA
• Codice UNSPSC: 41106514

Proprietà

• Nome del prodotto: AB-1 HA, 12070506
• Origine biologica: mouse mesothelium
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Epithelial; cells are small and can be spindly at low density but form a uniform cobblestone epithelial layer at near confluence
• Recettori: Not specified
• Malattie correlate: cancer

Descrizione

Origine della linea cellulare

Mouse mesothelioma cell line transfected with influenza haemagglutinin gene (HA)

Descrizione della linea cellulare

AB-1 HA is a mouse mesothelioma cell line transfected with influenza haemagglutinin gene (HA). Mice (BALB/c strain, female, 6-8 weeks old) were exposed to crocidolite asbestos through intraperitoneal injection, resulting in tumour development. Cultures were established from malignant mesothelial cells obtained from ascites fluid. The resulting celll ine, designated AB-1 was transfected with influenza haemagglutinin gene (HA) in order to generate an endogenous tumour antigen. Cell Bank Australia reference number: CBA1374.

Profilo DNA

Not specified

Terreno di coltura

RPMI 1640 (R0883) (with 2 mM L-Glutamine (G7513) + 25mM HEPES) + FBS / FCS (F2442) + 400ug/ml G418.

Mantenimento delle subcolture

Optimal split ratio 1:10 - 1:20 (seeding density 0.7 x 10⁴ cells/cm²). Cells incubated at 37 °C with 5% CO₂. Harvest the cells using 0.05% Trypsin/EDTA at 37 °C for 5 min. Cells will require passaging twice weekly at this seeding density.

Altre note

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Depositor and originator: Richard Lake - University of Western Australia, Australia