PNV35B

01080924, Reactivity: Anti-Osteoclasts (human foetal), ECV304 and U937 cells

• Codice UNSPSC: 41106514

Proprietà

• ImmunoDon: BALB/c mice
• Mieloma: Ag876
• Reattività: Anti-Osteoclasts (human foetal), ECV304 and U937 cells
• tecniche: cell culture | mammalian: suitable
• Isotipo: IgM plus IgG
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Anti-Osteoclastoma Hybridoma

Descrizione della linea cellulare

135B hybridoma was generated by fusing Ag876 myeloma cells with splenic cells of BALB/c mice immunised with tartrate-resistant acid phosphatase (TRAP) isolated from human osteoclastoma tumour. V35B antibody shows reactivity against the monocytic leukemia cell line U937, ECV304 cells and guinea pig tissue.

Immunogeno

Osteoclastoma tumour

Applicazioni

Immunocytochemistry

Terreno di coltura

RPMI 1640 (R0883) + 2mM L-Glutamine (G7513) + 10% FBS (F2442) + HT Supplement (H0137)

Mantenimento delle subcolture

Allow hybridomas to become 75% confluent prior to splitting. Seed at 3-9 x 100,000 cells/ml 5% CO₂, 37°C,

Altre note

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.