The term “dry” refers to the mesh range in the dry state. This product is hydrated and supplied as pre-swollen beads, which may appear moist or slurry-like. Should the resin dry out, rehydration is possible, provided the resin remains white. Do not use if the resin turns yellow, as this may indicate degradation. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/200/928/c7901pis.pdf
C7901
Chelex® 100
50-100 mesh (dry)
Sinonimo/i:
Iminodiacetic Acid Resin
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Cambia visualizzazione
| Voi/SKU | Disponibilità | Prezzo |
|---|---|---|
25 g | Per conoscere la disponibilità, visualizza il carrello | CHF 127.00 |
50 g | Per conoscere la disponibilità, visualizza il carrello | CHF 215.00 |
100 g | Per conoscere la disponibilità, visualizza il carrello | CHF 419.00 |
500 g | Per conoscere la disponibilità, visualizza il carrello | CHF 1510.00 |
Informazioni su questo articolo
CHF 127.00
Per conoscere la disponibilità, visualizza il carrello
Servizio Tecnico
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beads
Quality Segment
matrix
1% cross-linked polystyrene, cross-linked polystyrene beads
matrix active group
iminodiacetic acid (sodium form), paired iminodiacetate groups
matrix attachment
through methyl group to aromatic rings
particle size
50-100 mesh (dry)
pH
4-14
capacity
≥0.3 meq/mL binding capacity, 0.6 meq/g binding capacity (heavy metal ions)
Application
La resina Chelex® 100 in forma sodica è stata usata:
- per l′estrazione di DNA da campioni di ape
- per rimuovere estratti di fumo di sigaretta (CSE) e studiare l′effetto dell′espressione di epcidina nei polmoni murini
- per l′estrazione di DNA da colture batteriche
Il Chelex viene utilizzato nella cromatografia di affinità, cromatografia proteica, nelle resine chelanti, nelle resine di affinità e nei bioreagenti core.
Biochem/physiol Actions
Chelex® 100 è un copolimero a base di stirene-divinilbenzene contenente ioni imminodiacetato accoppiati, che agiscono da gruppi chelanti legando ioni metallici polivalenti. È considerato una resina debolmente acida. Chelex agisce come resina chelante utilizzata nell′estrazione di DNA da campioni forensi, come capelli, macchie di sangue raccolte su carte da filtro speciali o tamponi buccali.
Other Notes
Il prodotto è autoclavabile in forma sodica. La sua massima temperatura d′uso è di 75°C ed è insolubile in acqua.
Legal Information
Chelex is a registered trademark of Bio-Rad Laboratories, Inc.
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Questo articolo | |||
|---|---|---|---|
| description 50-100 mesh (dry) | description 100-200 mesh particle size | description 200-400 mesh particle size | description - |
| particle size 50-100 mesh (dry) | particle size 100-200 mesh | particle size 200-400 mesh | particle size 50-100 mesh |
| form beads | form crystals | form powder or chunks | form beads |
| pH 4-14 | pH - | pH - | pH - |
| matrix cross-linked polystyrene beads, 1% cross-linked polystyrene | matrix polystyrene/DVB | matrix polystyrene/DVB | matrix styrene-divinylbenzene (gel) |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| matrix attachment through methyl group to aromatic rings | matrix attachment - | matrix attachment - | matrix attachment - |
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Classe di stoccaggio
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Bonjour, pourriez-vous m'expliquer pourquoi vous indiquez le mot "dry" sur le nom de la résine Chelex100 alors que le fabricant, qui est Bio-Rad, indique que son aspect est liquide dans ses FDS ?
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I want to use this resin to extract the salts and retain the metals for analysis of the metal content in seawater samples. Is there any documentation on how to use the solvent and pH?
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This product has been used for trace metal studies, but the application has not been validated internally. Usage instructions can be reviewed in the product datasheet available at: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/200/928/c7901pis.pdf
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Can this resin be used with organic solvents (such as DMF, DMSO) in order to remove Cu ? For example in solid-phase peptide synthesis?
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The base matrix of the resin is styrene divinylbenzene which is chemically compatible with organic solvents at low concentrations.
Chelex 100 resin is not damaged by 100% organics, but it IS affected.
1) The functional group will lose its polarity and metals won't exchange onto the resin:
2) Chelex 100 resin will not work as an ion exchanger in 100% organic solvent.
3) In studies, Chelex 100 resin removed metal ions well in either 20 or 40% ethanol. End users should verify the efficiency of metal ion removal with the sample being treating. Perhaps doing a specific assay for the metal ions would help quantitate the metal content of the buffers before and after treatment. A batch overnight treatment of the solutions would remove metal ions more efficiently than a one hour treatment, particularly in the ethanol solutions.
Note, ethanol will shrink the resin beads and decrease the porosity of the resin, and this would decrease the efficiency of the exchange, particularly for larger metallic ions. Thus a solution of 40% ethanol will require a longer incubation time to remove all of the metal ions. Increasing the Chelex treatment time would allow more time for ion exchange to occur.
Stryrene divinylbenzene and iminodiacetic acid (the functional group) can leach from the resin, and this would be increased with the higher concentration of ethanol. The recommendation is to wash the resin with 100% ethanol and then equilibrate in aqueous solution immediately before using the resin. If the failure to aggregate is due to something leaching from the resin, this experiment should eliminate this possibility.
Also, the carboxyl groups on the resin have a very high selectivity for hydrogen ions, and will exchange hydrogen ions from water, resulting in an increased pH. There is no solution for this except to try equilibrating the resin using the protocol in the instruction manual (this doesn't work very well) or to adjust the pH of the buffer after the Chelex treatment.. The functional group will lose its polarity and metals won't exchange onto the resin. Studies have indicated the resin is still functional in 20% methanol and ethanol.
Organic solvents such as DMSO or DMF might also affect the selectivity of the resin slightly for divalent cations and would reduce the interaction between small hydrophobic molecules and the resin matrix. The resin may also swell upon exposure to the chemicals, but it won't melt or dissolve.
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Can this be used to remove calcium from FBS for Keratinocyte culture ?
1 answer-
This product has been used to remove calcium from FBS. However, the application has not been validated internally.
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How is the Chelex 100 resin used and how is it washed back? Can you please provide a manual of its usage?
1 answer-
Please see the link below to review the washing steps and usage instructions provided in the product datasheet.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/200/928/c7901pis.pdfHelpful?
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What is the grade (analytical; technical; molecular biology, or... ) of this reagent?
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This product is not assigned a specific grade or tested for a specific application.
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What solvent should I use to swell the Product C7901, Chelex 100 at the beginning?
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We recommend using a good grade of water (deionized or distilled).
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What is the particle size of the resin in Product C7901, Chelex 100?
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The particle size for this resin is 45-165 microns. The mean particle size is 90 microns.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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My Chelex 100 (Product C7901) in my column dried out. Can I still use it?
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The resin may be rehydrated and used, as long as it remains white. If the color of the re-swollen resin is yellow, do not use it.
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