C6423

α-Chymotrypsin from bovine pancreas

suitable for protein sequencing, salt-free, lyophilized powder

• Numero CAS: 9004-07-3
• Classificazione EC (Enzyme Commission): 3.4.21.1 (BRENDA, IUBMB)
• Numero CE: 232-671-2
• Numero MDL: MFCD00130481
• Codice UNSPSC: 12352204
• eCl@ss: 42010112
• NACRES: NA.26

Proprietà

• Grado: Proteomics Grade
• Livello qualitativo: 200
• Forma fisica: salt-free, lyophilized powder
• PM: 25 kDa
• Compatibilità: suitable for protein sequencing
• N° accesso UniProt: P00767
• Temperatura di conservazione: 2-8°C
• Informazioni sul gene: cow ... CTRB1(618826)

Descrizione

Descrizione generale

α-Chymotrypsin from bovine pancreas (bovine pancreatic α-chymotrypsin, CHT) is an enzyme protein. The influence of varying concentrations of organic solvents like ethanol, 1,4-dioxane and acetonitrile on CHT has been reported.

Chymotrypsin (Chy) is a serine protease. It corresponds to a molecular weight of 25.7 kDa and is widely used in pharmaceutical industry. It is synthesized in pancreas from chymotrypsinogen and require calcium for this conversion.

Applicazioni

α-Chymotrypsin from bovine pancreas is used for the following applications:

• Protein Identification by mass spectrometry (MS)
• The isolation and characterization of myosin heavy chains
• Toxin preparation
• The incubation of infected and uninfected cells for analysis of cellular proteins by SDS-PAGE

α-Chymotrypsin from bovine pancreas (BPC) may be used as a catalyst in the preparation of tetrahydroquinoline derivatives by Povarov reaction.

Azioni biochim/fisiol

α-Chymotrypsin from bovine pancreas is stabilized by Ca²⁺ and catalyzes the hydrolysis of peptide bonds in particular the amino acids tyrosine, phenylalanine, tryptophan, and leucine at their C-terminal side. α-Chymotrypsin is inhibited by Cu²⁺ and Hg.

A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond.

Definizione di unità

One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.

Altre note

View more information on chymotrypsin at www.sigma-aldrich.com/enzymeexplorer

Informazioni sulla sicurezza

• Pittogrammi: GHS08,GHS07,GHS09
• Avvertenze: Danger
• Indicazioni di pericolo: H302,H315,H319,H334,H335,H400
• Consigli di prudenza: P261 - P264 - P273 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Classi di pericolo: Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Organi bersaglio: Respiratory system
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Dispositivi di protezione individuale: dust mask type N95 (US), Eyeshields, Faceshields, Gloves