This is a product of PromoCell. Please see the instructions for use provided by the manufacturer:
1. Fill Lymphocyte Separation Medium 1077 in a tube. Use 3 ml for a 15 ml tube and 20 ml for a 50 ml tube.
2. Hold the tube at a 45° angle and carefully load the cell suspension to be separated on top of the separation medium.
Note: Perform this step slowly but constantly. Take care that the two phases do not mix and a sharp border is present.
For best results, use an electrical pipet aid with EX-function allowing the slow and constant efflux of the cell suspension from the pipet.
3. Centrifuge the tube at 440 x g for 40 minutes at room temperature without brake.
4. Carefully aspirate most of the supernatant without disturbing the layer of mononuclear cells in the interphase.
5. Aspirate the ring of mononuclear cells from the interphase using a pipet. Keep the volume as small as possible.
6. Wash the mononuclear cells with PBS/0.1% HSA or BSA and centrifuge at 360 x g for 10 minutes.
7. Wash the mononuclear cells with PBS/0.1% HSA or BSA and centrifuge at 200 x g for 10 minutes X 2
8. The mononuclear cell preparation is ready.
C-44010
Lymphocyte Separation Medium 1077
500 ml
Sinonimo/i:
Lymphocyte Medium, Separation Medium for Lymphocytes
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Cambia visualizzazione
| A voi/SKU | Disponibilità | Prezzo |
|---|---|---|
500 mL | Per conoscere la disponibilità, visualizza il carrello | CHF 118.00 |
Informazioni su questo articolo
NACRES:
NA.71
UNSPSC Code:
12352207
Servizio Tecnico
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Permettici di aiutartiform
aqueous solution
packaging
pkg of 500 ml
technique(s)
cell culture | mammalian: suitable
shipped in
ambient
storage temp.
room temp
General description
Lymphocyte Separation Medium 1077
Application
Lymphocyte Separation Medium 1077 is intended for the separation of the portion of vital mononuclear cells from whole blood, buffy coats, bone marrow and several other starting materials, e.g. crude cell preparations, by means of low density gradient centrifugation. Lymphocyte separation Medium has a density of 1.077 g/ml at 20°C. It is sterile, ready-to use and based on Ficoll 400 and sodium diatrizoate providing optimal physiological parameters and low cytotoxicity.
Preparation Note
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Lymphocyte Separation Medium 1077 is light-sensitive! Store the medium at room temperature in the dark immediately after arrival. Do not freeze. If stored properly, the product is stable until the expiry date stated on the label.
Analysis Note
All lots of PromoCell Lymphocyte Separation Medium 1077 are subjected to comprehensive quality control tests. Each lot is routinely tested for function and physical parameters such as osmolality and pH level. Approved in-house lots are used as a reference. In addition, all lots have been tested for the absence of microbial contaminants (fungi, bacteria).
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Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Classe di stoccaggio
12 - Non Combustible Liquids
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What's the procedure of lymphocytes separation with this media and the quantity used .
1 answer-
Helpful?
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What percentage of lymphocytes/monocytes, etc. would be expected if they use it on fresh human blood?
1 answer-
When utilizing the Lymphocyte Separation Medium 1077 (cat. #C-44010) on fresh human whole blood, the expected percentage of lymphocytes/monocytes typical for this sample is usually in the range of 70-90% of the enriched PBMCs.
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