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Merck
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Key Documents

C-43080

Sigma-Aldrich

NCCD Reagent

2 ml

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About This Item

Codice UNSPSC:
12352207
NACRES:
NA.71

Forma fisica

aqueous solution

tecniche

cell culture | mammalian: suitable

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

Descrizione generale

NCCD Reagent

Applicazioni

NCCD Reagent is intended exclusively for use in combination with the PromoCell Primary Cancer Cell Culture Medium D-ACF (C-28081). It allows for the culture vessel surface pre-treatment which is critical for the functionality of the Primary Cancer Cell Culture System.

Qualità

All lots of PromoCell NCCD Reagent are subjected to comprehensive quality control tests. Each lot is routinely tested for functionality using a human cancer cell line in conjunction with the Primary Cancer Culture Medium D-ACF. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Nota sulla preparazione

The NCCD Reagent is provided as a 20x stock and must be diluted to a working concentration before use. One 2 ml vial of NCCD Reagent yields 40 ml of ready-to-use working solution sufficient to treat 400 cm2 of culture surface, e.g. seven complete 6-well plates.Dilute the thawed NCCD Reagent stock solution 1:20 with PBS w/o Ca2+/Mg2+. Use 100 uL per cm2 of culture surface to treat the tissue culture vessel with the diluted NCCD Reagent and leave the closed vessel for at least 1 hour at RT. Make sure that the NCCD covers the entire vessel surface. Aspirate the NCCD solution just before seeding the cells.

Stoccaggio e stabilità

Store at -20°C immediately after arrival. If stored properly, the product is stable until the expiry date stated on the label. Unless used immediately, the sealed vessel containing the NCCD Reagent may be stored for up to 3 months at 2 to 8°C for later use. Diluted NCCD Reagent solution may be stored for up to 4 weeks at 2 to 8°C. Protect from light.

Mantenimento delle subcolture

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

nwg


Certificati d'analisi (COA)

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Contenuto correlato

The PromoCell Primary Cancer Culture System allows selective isolation of malignant cancer cells from tumor associated stromal cells in patient derived xenographs (PDX models) and tumor samples.

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