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C-39439

Sigma-Aldrich

Adipocyte Nutrition Medium

SupplementPack containing all media supplements as individual vials, 1 Pack for 500 ml

Sinonimo/i:

Recommended with C-27431

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About This Item

Codice UNSPSC:
12352207
NACRES:
NA.71

Forma fisica

liquid

Confezionamento

pkg of 1 kit

tecniche

cell culture | mammalian: suitable

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

Descrizione generale

Adipocyte Nutrition Medium SupplementPack (basal medium sold separately)

To form a complete medium, it should be combined with the basal medium: C-27431

Applicazioni

Adipocyte Nutrition Medium can be used to cultivate mature adipocytes. The medium is optimized for primary human cells but can also be used for murine adipocytes.Recommended for:Human Adipocytes

Componenti

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

Qualità

All lots of PromoCell Preadipocyte/Adipocyte Media are subjected to comprehensive quality control tests using primary human white preadipocytes. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested preadipocytes. In addition, the Preadipocyte Differentiation Medium/Adipocyte Nutrition Medium are tested for the capacity to induce the terminal differentiation of preadipocytes into mature adipocytes. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Nota sulla preparazione

Final supplement concentrations after addition to the basal medium:Fetal Calf Serum: 0.03 ml / mld-Biotin: 8 µg / mlInsulin (recombinant human): 0.5 µg / mlDexamethasone: 400 ng / ml

Stoccaggio e stabilità

Store the SupplementPack at -20°C in the dark immediately after arrival. If stored properly, it is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Mantenimento delle subcolture

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Raccomandato

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Determinazione del prezzo

Pittogrammi

FlameExclamation mark
GHS02,GHS07

Avvertenze

Danger

Indicazioni di pericolo

H225,H319

Classi di pericolo

Eye Irrit. 2 - Flam. Liq. 2

Codice della classe di stoccaggio

3 - Flammable liquids

Punto d’infiammabilità (°F)

55.4 °F

Punto d’infiammabilità (°C)

13 °C

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Articoli

Primary Human Preadipocytes and Preadipocyte/Adipocyte Growth & Differentiation Media

Primary human white preadipocytes and culture media for the expansion of undifferentiated preadipocytes, differentiation to adipocytes, and cultivation of mature adipocytes. Protocols for handling, cell passaging, media, and product use.

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