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C-23110

Sigma-Aldrich

Fibroblast Growth Medium

Kit including Basal Medium and SupplementPack, 500 ml

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About This Item

Codice UNSPSC:
12352207
NACRES:
NA.71

Forma fisica

liquid

Confezionamento

pkg of 500 ml

tecniche

cell culture | mammalian: suitable

Condizioni di spedizione

wet ice

Descrizione generale

Fibroblast Growth Medium Kit

Applicazioni

The Fibroblast Growth Medium is optimized for the cultivation of neonatal and juvenile fibroblasts. The medium is optimized for primary human cells, but can also be used for bovine, porcine, and rat fibroblasts as well as fibroblast cell lines. Recommended for: Normal Human Dermal Fibroblasts (NHDF), juvenile foreskin.

Componenti

Part of a Cell Culture System offered in a variety of formats: Growth Medium (Ready-to-use): Includes Basal Medium and Supplement Mix. The Growth Medium Kit: Includes Basal Medium and Supplement Pack. The Supplement Mix: Contains all media supplements premixed in one vial. The Supplement Pack: Contains all media supplements as individual vials. While the supplements of the Fibroblast Growth Medium are serum-free, the Basal Medium is manufactured with 0.5% serum.

Qualità

All lots of PromoCell Fibroblast Growth Medium are subjected to comprehensive quality control tests using primary human fibroblasts. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested fibroblasts. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Nota sulla preparazione

Final supplement concentrations after addition to the basal medium:Basic Fibroblast Growth Factor (recombinant human): 1 ng / ml Insulin: 5 µg / ml

Stoccaggio e stabilità

Store the Basal Medium at 4 to 8°C in the dark and the SupplementMix or SupplementPack at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Mantenimento delle subcolture

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Codice della classe di stoccaggio

10 - Combustible liquids

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Articoli

Primary Human Fibroblasts and Growth Media

Primary human fibroblasts and growth medium for the in vitro cultivation of juvenile and adult fibroblasts. Protocols for handling, cell passaging, media, and product use.

Protocolli

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3D cell culture protocol for generating epidermal human skin tissue using primary human keratinocytes, dermal fibroblasts, and collagen-coated transwell inserts.

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