PromoCell’s human pericytes derived from microvessels of the human placenta are cryopreserved at the end of secondary culture (P1) and after thawing are considered P2. The number of populations doublings is not determined for each individual cell lot, however they are guarenteed to be grown for at least 15 population doublings.
C-12980
Human Pericytes from Placenta (hPC-PL)
500,000 cryopreserved cells
Sinonimo/i:
Pericyte Culture
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
Scegli un formato
Cambia visualizzazione
1 EACH
CHF 1’450.00
About This Item
Codice UNSPSC:
41106514
NACRES:
NA.81
Prodotti consigliati
Origine biologica
human placenta
Confezionamento
pkg of 500,000 cells
Morfologia
( pericytes)
tecniche
cell culture | mammalian: suitable
Condizioni di spedizione
dry ice
Temperatura di conservazione
−196°C
Descrizione generale
Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.
Origine della linea cellulare
Placenta
Applicazioni
Human Pericytes are isolated from human placenta tissue and provided in a cryopreserved format. Pericytes are multipotent mesenchymal-like cells found in association with small blood vessel walls. They are important for angiogenesis, the structural integrity of the microvasculature, and blood flow regulation. However, they can also develop into malignant tumors called hemangiopericytomas. To date, pericytes have demonstrated the ability to differentiate into adipocytes, osteoblasts, fibroblasts, smooth muscle cells, and phagocytes (macrophages).
Qualità
Rigid quality control tests are performed for each lot of Pericytes. The cells are tested for morphology, adherence rate, and viability. Furthermore, they are characterized by flow cytometric analysis for a comprehensive panel ofmarkers, e.g. CD31, CD34, CD105, and CD146. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, and microbial contaminants (fungi, bacteria, and mycoplasma).
Attenzione
Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.
Mantenimento delle subcolture
Click here for more information.
Altre note
Recommended Plating Density: 4000 cells per cm2Tested markers: CD146+, CD105+, CD31-, CD34-
Prodotti consigliati
Recommended Primary Cell Culture Media:Link
Esclusione di responsabilità
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Scegli una delle versioni più recenti:
Certificati d'analisi (COA)
Lot/Batch Number
It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documenti section.
Se ti serve aiuto, non esitare a contattarci Servizio Clienti
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Articoli
What are pericytes? What are common pericyte markers? Are pericytes mesenchymal stem cells? Learn more about pericytes in our FAQ page. Explore over 350 PromoCell products.
Primary human pericytes and cell culture media optimized for the in vitro cultivation of human pericytes from all tissues. Protocols for handling, cell passaging, media, and product use.
Smooth muscle human primary cells isolated from blood vessels and hollow organs, plus smooth muscle cell growth media. Protocols on media handling, cell passaging and product use.
Protocolli
Cell culture protocol: the endothelial cell transwell migration and invasion assay used to study angiogenesis and cancer cell metastasis. Explore over 350 PromoCell products.
Contenuto correlato
Cell culture protocol: the endothelial tube formation assay to study angiogenesis using HUVECs and other endothelial cell types. Explore over 350 PromoCell products.
-
How long do pericytes grow in culture?
1 answer-
Helpful?
-
-
What markers are used to characterize pericytes?
1 answer-
Pericytes are positive for CD146, PDGFRβ, αSMA, CD13, NG2, Desmin, and negative for CD31, CD34, CD45, and CD56.
Helpful?
-
-
How are pericytes isolated?
1 answer-
Pericyte isolation generally starts with enzymatic digestion of tissue with Trypsin EDTA, usually followed by the isolation of microvessel fragments via successive filtration steps. Pure pericyte cultures can be obtained through either positive or negative immuno-selection via the use of magnetic beads or flow cytometry using the antibody markers above.
Helpful?
-
Active Filters
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.
