C-12380

Human Aortic Adventitial Fibroblasts (HAoAF)

500,000 cryopreserved cells

• NACRES: NA.81
• UNSPSC Code: 41106514
• Biological source: human aorta
• Morphology: (fibroblast)
• Relevant disease(s): hypertension; cardiovascular diseases

Proprietà

• biological source: human aorta
• product line: PromoCell^®
• packaging: pkg of 500,000 cells
• morphology: (fibroblast)
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): hypertension; cardiovascular diseases
• shipped in: dry ice
• storage temp.: −196°C

Descrizione

General description

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Application

Primary Human Aortic Adventitial Fibroblasts (HAoAF) are isolated from human aortic adventitial tissue and are provided in a cryopreserved format. HAoAF can be used for studies concerning vascular diseases e.g. neointimal formation, vascular remodeling, atherosclerosis, and hypertension. They are also often used for the examination of fibroblast disorders like fibrosis or other diseases linked to either imperfect or excessive accumulation of fibroblasts.

Biochem/physiol Actions

Aorta

Preparation Note

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Analysis Note

Rigid quality control tests are performed for each lot of Human Fibroblasts. They are tested for cell morphology, adherence rate and cell viability. Flow cytometric analyses of the fibroblast specific antigen CD90 are performed for each lot. Growth performance is tested through multiple passages up to 10 or 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Other Notes

Recommended Plating Density: 3500 - 7000 cells per cm²Passage After Thawing: P2Tested Markers: CD90 positiveGuaranteed population doublings: >10

Recommended Primary Cell Culture Media:Link

Legal Information

PromoCell is a registered trademark of PromoCell GmbH

Disclaimer

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Informazioni sulla sicurezza

• Classe di stoccaggio: 12 - Non Combustible Liquids
• Che succede?: WGK 1
• Punto di infiammabilità (°F): Not applicable
• Punto di infiammabilità (°C): Not applicable