C-12285

Human Cardiac Microvascular Endothelial Cells (HCMEC)

500,000 cryopreserved cells

• Sinonimo/i: Human endothelial cells
• NACRES: NA.81
• UNSPSC Code: 41106514
• Biological source: human heart (ventricles)
• Morphology: ( endothelial)
• Relevant disease(s): diabetes; hypertension; ischemia/reperfusion injury

Proprietà

• biological source: human heart (ventricles)
• product line: PromoCell^®
• packaging: pkg of 500,000 cells
• morphology: ( endothelial)
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): diabetes; hypertension; ischemia/reperfusion injury
• shipped in: dry ice
• storage temp.: −196°C

Descrizione

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Application

Primary Human Cardiac Microvascular Endothelial Cells (HCMEC) are isolated from heart ventricles from a single donor and are provided in a cryopreserved format. The cells are routinely analyzed by immunofluorescent staining: they stain positive for CD31 and Willebrand factor and negative for smooth muscle alpha-actin. HCMEC intensively interact with cardiomyocytes and therefore have a characteristic phenotype different from other microvascular endothelial cells. HCMEC play an important role in the physiological regulation of coronary blood flow and capillary exchange. Medical conditions such as hypertrophy, ischemia, hypertension, and diabetes mellitus are associated with endothelial dysfunction.

Biochem/physiol Actions

Heart

Preparation Note

Subculture Routine –Click here for more information.

Analysis Note

Rigid quality control tests are performed for each lot of Microvascular Endothelial Cells. They are tested for cell morphology and cell type specific markers, e.g. von Willebrand Factor (vWF), CD31 and Podoplanin using flow cytometric analyses. Growth performance is tested through multiple passages up to 15 population doublings (PD) without antibiotics or antimycotics. In addition, all cells have been tested forthe absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Other Notes

Recommended Plating Density: 10000 - 20000 cells per cm²Passage After Thawing: P2Tested Markers: von Willebrand Factor (vWF) positive, CD31 positive, Dil-Ac-LDL uptake positiveGuaranteed population doublings: >15

Recommended Primary Cell Culture Media:Link

Legal Information

PromoCell is a registered trademark of PromoCell GmbH

Disclaimer

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Informazioni sulla sicurezza

• Classe di stoccaggio: 12 - Non Combustible Liquids
• Che succede?: WGK 1
• Punto di infiammabilità (°F): Not applicable
• Punto di infiammabilità (°C): Not applicable