C0817

Monoclonal Anti-HLA-DQ antibody produced in mouse

clone HK19, purified immunoglobulin, buffered aqueous solution

• Sinonimo/i: Monoclonal Anti-HLA-DQ
• Codice UNSPSC: 12352203

Proprietà

• Origine biologica: mouse
• Coniugato: unconjugated
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: HK19, monoclonal
• Stato: buffered aqueous solution
• Reattività contro le specie: human
• tecniche: flow cytometry: 5 μL using 1 × 10⁶ cells
• Isotipo: IgG2b
• N° accesso UniProt: P01920
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: 2-8°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... HLA-DQB1(3119)

Descrizione

Specificità

Recognizes MHC Class II molecules that are preferentially expressed on the surface of B lymphocytes. The antibody reacts weakly with resting or ConA-activated T cells, monocytes, granulocytes, and erythrocytes.

Immunogeno

GM-1500 human tumor cell line.

Applicazioni

Monoclonal Anti-HLA-DQ antibody produced in mouse is suitable for flow cytometry at a concentration of 5 μL using 1 × 10⁶ cells.

Azioni biochim/fisiol

Human leukocyte antigen (HLA)-DQ is a cell surface receptor type protein found on antigen presenting cells. DQ is a αβ heterodimer of the MHC Class II type. It plays a central role in the immune system and is expressed in antigen presenting cells. HLA-DQ expression in cystic fibrosis CF is associated with inflammation and reduces response to Interferon γ (IFNγ). HLA-DQ acts as alloantibody most frequently associated with the generation of de novo donor-specific antibody (DSA) and antibody-mediated-rejection but does not support organ transplantation.

Descrizione del bersaglio

HLA-DQ molecules are bimolecular glycoprotein complexes of 29 kDa and 33 kDa components. The HLA-DQ antigens are present on peripheral blood lymphocytes and tonsillar and splenic mononuclear cells.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide