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B0174

Sigma-Aldrich

AccuTaq LA 10× Buffer

10X Buffer for long and accurate PCR

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About This Item

Codice UNSPSC:
41106306
Prezzi e disponibilità al momento non sono disponibili

Grado

Molecular Biology
for molecular biology

Livello qualitativo

Stato

liquid

Confezionamento

vial of 0.5 mL

Concentrazione

10 ×

tecniche

PCR: suitable

Temperatura di conservazione

−20°C

Descrizione generale

AccuTaq LA 10X buffer is optimized for enhanced amplification of complex templates, when using AccuTaq long and accurate (LA) DNA polymerase.

Applicazioni

AccuTaq LA 10× Buffer has been used:

  • in PCR reaction mixture for the amplification of human BRCA1 gene.[1]
  • as a component of the PCR mix for the amplification of the genomic DNA from Triticum aestivum[2]
  • as a component of the PCR mix for the reverse transcription of RNA from foot and mouth disease virus (FMDV)[3]

Note legali

AccuTaq is a trademark of Sigma-Aldrich Co. LLC

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Descrizione
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Indicazioni di pericolo

Consigli di prudenza

Classi di pericolo

Aquatic Chronic 3

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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Hameer Ruparel et al.
Genome research, 14(2), 296-300 (2004-02-06)
Deletion or insertion mutations lead to a frameshift that causes misalignment between wild-type and mutated allele sequences, making it difficult to identify such mutations unambiguously by using electrophoresis-based DNA sequencing. We have previously established the feasibility of an accurate DNA
Vandana Jaiswal et al.
PloS one, 10(6), e0129400-e0129400 (2015-06-16)
TaGW2 is an orthologue of rice gene OsGW2, which encodes E3 RING ubiquitin ligase and controls the grain size in rice. In wheat, three copies of TaGW2 have been identified and mapped on wheat homoeologous group 6 viz. TaGW2-6A, TaGW2-6B
Arunava Sarkar et al.
Biologicals : journal of the International Association of Biological Standardization, 48, 10-23 (2017-06-25)
In this study, the efficiency of binary ethyleneimine (BEI) in combination with formaldehyde (FA) and glutaraldehyde (GTA) in inactivating the Indian FMDV vaccine strains is compared. The acceptable safety of virus inactivation was faster and the inactivation rates were increased
Eva-Maria Ladenburger et al.
Molecular and cellular biology, 29(13), 3605-3622 (2009-04-22)
A database search of the Paramecium genome reveals 34 genes related to Ca(2+)-release channels of the inositol-1,4,5-trisphosphate (IP(3)) or ryanodine receptor type (IP(3)R, RyR). Phylogenetic analyses show that these Ca(2+) release channels (CRCs) can be subdivided into six groups (Paramecium
Jeong-Yun Choi et al.
The Journal of biological chemistry, 286(36), 31180-31193 (2011-07-26)
The hyperthermophilic crenarchaeon Sulfolobus solfataricus P2 encodes three B-family DNA polymerase genes, B1 (Dpo1), B2 (Dpo2), and B3 (Dpo3), and one Y-family DNA polymerase gene, Dpo4, which are related to eukaryotic counterparts. Both mRNAs and proteins of all four DNA

Protocolli

REDAccuTaq LA protocol offers high-fidelity amplification of long PCR fragments with direct gel loading capability.

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