AB0300
Alkaline Phosphatase Blue Membrane Substrate Solution
alkaline phosphatase substrate, solution
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About This Item
Codice UNSPSC:
12352204
eCl@ss:
42010105
NACRES:
NA.83
Prodotti consigliati
Nome del prodotto
Alkaline Phosphatase Blue Membrane Substrate Solution, sufficient for 400 mL working substrate
Stato
solution
Livello qualitativo
impiego
sufficient for 400 mL working substrate
Temperatura di conservazione
2-8°C
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Descrizione generale
Applicazioni
Alkaline Phosphatase Blue Membrane Substrate Solution has been used:
- to analyse direct alkaline phosphatase (AP) activity[3]
- to perform alkaline phosphatase assays with colorimetric system[4]
- to analyse direct alkaline phosphatase (AP) activity of the induced pluripotent stem cells (iPSC) line LND554SV.3[5][6]
Prior to reaction with alkaline phosphatase, the BCIP® reagent is colorless to faint blue and the Nitro BT reagent is yellow. The two-component mixture develops a bluish-purple product with alkaline phosphatase in membrane-type assays. This substrate is not recommended for ELISA assays.
Stato fisico
A 2 component kit sufficient for preparing 400 mL of working substrate.
Supplied as a two-component buffered alkaline phosphatase substrate containing bromochloroindolyl phosphate (BCIP®) and Nitro Blue Tetrazolium (NBT/Thiazolyl Blue/Nitro BT)
Note legali
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
Codice della classe di stoccaggio
10 - Combustible liquids
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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I clienti hanno visto anche
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Al-Rashida M and Iqbal J
Mini-Reviews in Medicinal Chemistry, 15(1), 41-51 (2015)
Generation of a human iPSC line from a patient with Leigh syndrome
Galera T, et al.
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Giulia Coppiello et al.
Stem cell research, 21, 47-50 (2017-07-06)
We generated a rat iPSC line called ATCi-rSD95 from transgenic Sprague-Dawley GFP fetal fibroblasts. Established ATCi-rSD95 cells present a normal karyotype, silencing of the transgenes and express pluripotency-associated markers. Additionally, ATCi-rSD95 cells are able to form teratoma with differentiated cells
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Journal of Cardiovascular Translational Research, 4(2), 121-130 (2011)
Articoli
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
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