This product is not fatty acid free. Currently, a fatty acid free version of this material is not available.
A9771
Albumin–fluorescein isothiocyanate conjugate
protein bovine
Sinonimo/i:
FITC-albumin
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Informazioni su questo articolo
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Servizio Tecnico
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Permettici di aiutartibiological source
protein from bovine
Quality Segment
form
powder
extent of labeling
≥7 mol FITCmol albumin
technique(s)
ELISA: suitable
solubility
10 mM Tris pH 7.0: 10 mg/mL
application(s)
detection
storage temp.
2-8°C
General description
Albumin makes up 60% of the plasma protein, which is produced by the liver. It regulates colloid osmotic pressure. Albumin is implicated in the transportation, distribution and metabolism of fatty acids, thyroid hormones and drugs. It reduces weight gain, blocks pulmonary edema, decreases ascites and lowers tissue edema.
Application
Albumin−fluorescein isothiocyanate conjugate has been used:
- as a control to compare the intracellular delivery efficiency of proteins with or without carrier
- for the fluorescent visualization of proteins and cells
- as a blocking agent in ELISA
- as a chemical for brain perfusion and fixation
- for in vivo capillary–epithelial permeability experiment
- for confocal microscopy of right ventricular (RV) vasculature in rats
Preparation Note
Prepared from crystallized and lyophilized albumin (A 4378).
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Questo articolo | |||
|---|---|---|---|
| technique(s) ELISA: suitable | technique(s) ELISA: suitable | technique(s) SDS-PAGE: suitable | technique(s) titration: suitable |
| solubility 10 mM Tris pH 7.0: 10 mg/mL | solubility water: soluble (50 mg/ml) | solubility H2O: soluble 50 mg/mL | solubility acetone: soluble 10 mg/mL |
| application(s) detection | application(s) - | application(s) - | application(s) diagnostic assay manufacturing |
| form powder | form lyophilized powder | form lyophilized powder | form powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Classe di stoccaggio
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Is this albumin "Fatty Acid Free"?
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Is the conjugate chromophore based 5-fluorescein, 6-fluorescein, or is based on an isomeric (5/6-) mixture?
1 answer-
The chromaphore used to prepare this product is Fluorescein 5-isothiocyanate.
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How is the FITC bound to the albumin in Product A9771, Albumin-fluorescein isothiocyanate conjugate (FITC-albumin)?
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The FITC is bound through the epsilon amino group of the lysine amino acid residues. Each lot number will have a varying number of FITC molecules bound.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What are the excitation and emission maxima for Product A9771, Albumin-fluorescein isothiocyanate conjugate (FITC-albumin)?
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The excitation maximum is 495 nm and the emission maximum is 520 nm.
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Is there any free FITC present in Product A9771, Albumin-fluorescein isothiocyanate conjugate?
1 answer-
We would not expect there to be free FITC. The final product is dialyzed against distilled water using dialysis tubing with a molecular weight cutoff of 12,000 prior to it being lyophilized.
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