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A9253

L-Amino Acid Oxidase from Crotalus adamanteus

Type I (dried venom)

Sinonimo/i:

L-AAO, L-Amino acid: oxygen oxidoreductase (deaminating)

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Informazioni su questo articolo

Numero CAS:
9000-89-9
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-564-0
MDL number:
MFCD00131454
Numero CE:
1.4.3.2 (BRENDA, IUBMB)
Biological source:
Crotalus adamanteus

biological source

Crotalus adamanteus

Quality Segment

type

Type I (dried venom), Type I

form

powder

mol wt

~130 kDa

solubility

H2O: soluble 1.0 mg/mL, clear(lit.)

storage temp.

−20°C

General description

L-Amino acid oxidase (LAAO) is a flavoprotein with a molecular weight of 130 kDa. It consists of two different subunits of approximately 70 kDa. Each molecule of holoenzyme has two flavin adenine dinucleotide (FAD) molecules. LAAO is a glycoprotein containing about 2-5% carbohydrate, including sialic acid. optimum pH is approximately 7.5. It occurs in many snake venoms apart from microorganisms and animal tissue, especially in kidney. In the N-terminal region, it has a βαβ domain with glutamic acid residues. LAAO imparts yellow color to venom.

Application

L-Amino Acid Oxidase from Crotalus adamanteus has been used in the quantification of rid protein in imine deaminase activity.
L-amino acid oxidase (LAAO) is used to convert L-amino acids to their corresponding α-keto acids. L-amino acid oxidase, from Sigma, has been used in leucine aminopeptidase (LAP) activity assays. The enzyme has been immobilized and used in an enzymatic flow-injection procedure with chemiluminescence detection for on-site determination of L-alanine.

Biochem/physiol Actions

L-Amino acid oxidase (LAAO) from Crotalus adamanteus requires Mg2+ for its activation. The N-terminal region is essential for FAD binding. The H2O2 generated by LAAO based oxidation reactions lead to prevention of platelet aggregation and induces edema and apoptosis. LAAO activity is inhibited by ethylenediaminetetraacetic acid (EDTA) and phenylmethylsulfonyl fluoride (PMSF).

Preparation Note

Dissolves in water at 1 mg/mL concentration to form a clear solution.

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Pittogrammi

Skull and crossbones
GHS06

Parola di avvertenza

Danger

Codici di pericolo

H300 + H310 + H330

Hazard Classifications

Acute Tox. 1 Inhalation - Acute Tox. 2 Dermal - Acute Tox. 2 Oral

Classe di stoccaggio

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Che succede?

WGK 3

Punto di infiammabilità (°F)

Not applicable

Punto di infiammabilità (°C)

Not applicable

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Certificati d'analisi (COA)

Lot/Batch Number

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Questions

  1. What is the enzyme activity of this product?

    1 answer

    1. This activity of this product is not tested on a lot-to-lot basis. However, historical data indicate a range of 0.28 - 0.40 units per milligram.

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