A8181

Amido Black Staining Solution 2X

electrophoresis reagent

• Sinonimo/i: Naphthol Blue Black solution, Amido Black 10B
• Formula empirica (notazione di Hill): C₂₂H₁₄N₆Na₂O₉S₂
• Numero CAS: 1064-48-8
• Peso molecolare: 616.49
• Beilstein: 5374263
• Numero MDL: MFCD00004017
• Codice UNSPSC: 12171500
• ID PubChem: 24891210
• NACRES: NA.32

Proprietà

• Forma fisica: liquid
• Stringa SMILE: [Na+].[Na+].Nc1c(\N=N/c2ccc(cc2)[N+]([O-])=O)c(cc3cc(c(\C=C/c4ccccc4)c(O)c13)S([O-])(=O)=O)S([O-])(=O)=O
• InChI: 1S/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2/b26-25+,27-24+;;; AOMZHDJXSYHPKS-DROYEMJCSA-L

Descrizione

Descrizione generale

Amido black (AB) is an acidic dye used for staining proteins. It can detect proteins levels of 50ng in both polyvinylidene difluoride (PVDF) and nitrocellulose membrane. AB aids protein visualization even at low concentrations. It has applications in forensics due to its ability to stain blood proteins in fingerprints.

Applicazioni

Amido Black Staining Solution 2X has been used in the staining:

• serum proteins present in the culture supernatant
• cysteine sulfinic acid decarboxylase protein in mouse liver homogenate
• histones from chromatin samples

Ricostituzione

After dilution with deionized water to a final volume of 500 ml, solution will contain 0.1% (w/v) amido black, 25% (v/v) isopropanol and 10% (v/v) acetic acid.

Informazioni sulla sicurezza

• Pittogrammi: GHS02,GHS07
• Avvertenze: Danger
• Indicazioni di pericolo: H225,H315,H319,H336
• Consigli di prudenza: P210 - P233 - P240 - P241 - P303 + P361 + P353 - P305 + P351 + P338
• Classi di pericolo: Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2 - STOT SE 3
• Organi bersaglio: Central nervous system
• Codice della classe di stoccaggio: 3 - Flammable liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): 53.6 °F
• Punto d’infiammabilità (°C): 12 °C