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Documenti fondamentali

A8094

Sigma-Aldrich

L-arginina

98.5-101.0%, suitable for cell culture, BioXtra, non-animal source

Sinonimo/i:

Acido (S)-2-ammino-5-guanidinopentanoico

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10 MG
CHF 42.70
25 G
CHF 46.60
100 G
CHF 150.00
1 KG
CHF 947.00

CHF 42.70


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Cambia visualizzazione
10 MG
CHF 42.70
25 G
CHF 46.60
100 G
CHF 150.00
1 KG
CHF 947.00

About This Item

Formula condensata:
H2NC(=NH)NH(CH2)3CH(NH2)CO2H
Numero CAS:
Peso molecolare:
174.20
Beilstein:
1725413
Numero CE:
Numero MDL:
Codice UNSPSC:
12352209
eCl@ss:
32160406
ID PubChem:
NACRES:
NA.26

CHF 42.70


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Nome del prodotto

L-arginina, from non-animal source, meets EP, USP testing specifications, suitable for cell culture, 98.5-101.0%

Origine biologica

non-animal source

Livello qualitativo

agenzia

USP/NF
meets EP testing specifications
meets USP testing specifications

Nome Commerciale

BioXtra

Saggio

98.5-101.0%

Stato

powder

tecniche

cell culture | mammalian: suitable

Impurezze

endotoxin, tested

Colore

white

Punto di fusione

222 °C (dec.) (lit.)

Solubilità

H2O: 100 mg/mL

applicazioni

agriculture
peptide synthesis
pharmaceutical (small molecule)

Stringa SMILE

N[C@@H](CCCNC(N)=N)C(O)=O

InChI

1S/C6H14N4O2/c7-4(5(11)12)2-1-3-10-6(8)9/h4H,1-3,7H2,(H,11,12)(H4,8,9,10)/t4-/m0/s1
ODKSFYDXXFIFQN-BYPYZUCNSA-N

Informazioni sul gene

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Applicazioni

L-Arginine has been used:
  • as a Roswell park memorial institute medium (RPMI) media component in the isolation and culture of peripheral blood mononuclear cells (PBMCs)[1]
  • as a RPMI media component for tissue culture[2]
  • in DMEM medium for the identification and quantification of phosphorylation sites by stable isotope labeling by amino acids in cell culture (SILAC) and LCMS/MS[3]

Azioni biochim/fisiol

Substrato della sintasi del monossido di azoto, che viene convertita a citrullina e monossido di azoto (NO). Induce il rilascio di insulina con un meccanismo dipendente dal monossido di azoto.
L-Arginine is a conditionally essential amino acid, that play a vital role in the production of nitric oxide. It acts as a precursor for the synthesis of polyamines, agmatine and urea. L-Arginine is usually essential in children and non-essential in adults.[4]

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


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Certificati d'analisi (COA)

Lot/Batch Number

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Supelco

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L-arginina

Association of reactive oxygen and nitrogen intermediate and complement levels with apoptosis of peripheral blood mononuclear cells in lupus patients
Oates J C, et al.
Arthritis and Rheumatism, 56(11), 3738-3747 (2007)
Requirement of the mTOR Kinase for the Regulation of Maf1 Phosphorylation and Control of RNA Polymerase III-dependent Transcription in Cancer Cells
Boris Shor
The Journal of Biological Chemistry, 285(20), 15380-15392 (2010)
J. P. Felix D'Mello
Amino Acids in Human Nutrition and Health, 225-225 (2012)
Anti-aging effects of L-arginine
Gad M Z
Journal of Advanced Research, 1(3), 169-177 (2010)
Cell-specific Labeling Enzymes for Analysis of Cell?Cell Communication in Continuous Co-culture
Tape CJ, et.al.
Molecular and Cellular Proteomics, 13(7), 1866-1876 (2014)

Protocolli

Protocollo del saggio di proliferazione e vitalità cellulare e citotossicità con MTT. Istruzioni per la preparazione dei reagenti per il saggio con MTT con esempi di applicazione.

Perform colorimetric assays for nonradioactive quantification of cellular proliferation, viability, and cytotoxicity for adherent or suspension cells cultured in 96-well microplates.

MTT assay protocol for measuring cell viability, proliferation and cytotoxicity. Instructions for MTT reagent preparation and examples of applications.

Questions

1–2 of 2 Questions  
  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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