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A0483

Sigma-Aldrich

Monoclonal Anti-Actin (20-33) antibody produced in rabbit

clone SIG2-AC2, ascites fluid

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.41

Origine biologica

rabbit

Coniugato

unconjugated

Forma dell’anticorpo

ascites fluid

Tipo di anticorpo

primary antibodies

Clone

SIG2-AC2, monoclonal

PM

antigen ~42 kDa

Reattività contro le specie

chicken, rat, human

tecniche

immunocytochemistry: suitable
immunohistochemistry: suitable
indirect ELISA: suitable
indirect immunofluorescence: 1:400
western blot: 1:50-1:100 using chicken gizzard total extract

Isotipo

IgG

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... ACTB(60)

Descrizione generale

Monoclonal Anti-Actin (20-33) (rabbit IgG) is a rabbit monoclonal derived from the hybridoma SIG2-AC2 produced by the fusion of rabbit myeloma cells and splenocytes from rabbits immunized with a synthetic peptide. Actin is one of the most conserved eukaryotic proteins and is expressed in mammals and birds with at least six isoforms characterized by electrophoresis and amino acid sequence analysis. There are three α-actins (skeletal, cardiac and smooth muscle), one β-actin (β-non-muscle) and two γ-actins (γ-smooth muscle and g-non-muscle).

Immunogeno

synthetic peptide corresponding to amino acids 20-33 of actin, attached to a multiple antigen peptide (MAP) backbone.

Applicazioni

Monoclonal Anti-Actin (20-33) antibody produced in rabbit has been used in:
  • western blotting[1]
  • enzyme-linked immunosorbent assay (ELISA)
  • immunohistochemistry
  • immunocytochemistry

Stato fisico

Supplied as ascites fluid containing 15 mM sodium azide as a preservative.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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