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97875

Sigma-Aldrich

Atto 647

BioReagent, suitable for fluorescence, ≥90% (HPCE)

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About This Item

Numero MDL:
Codice UNSPSC:
12352108
NACRES:
NA.32

Nome Commerciale

BioReagent

Saggio

≥90% (HPCE)

Forma fisica

powder

Produttore/marchio commerciale

ATTO-TEC GmbH

Trasmittanza

254 nm
647 nm

Fluorescenza

λex 643 nm; λem 665 nm in 0.1 M phosphate pH 7.0

λ

in ethanol (with 0.1% trifluoroacetic acid)

Assorbanza UV

λ: 645-651 nm Amax

Compatibilità

suitable for fluorescence

Temperatura di conservazione

−20°C

Descrizione generale

Atto 647 is a zwitterionic dye with a net electrical charge of zero. It is a pH sensitive product. While practically stable up to pH 8.5, it slowly degrades at higher pH. Atto 647 is a new label with high molecular absorption (120,000) and quantum yield (0.20) as well as enough Stokes shift between excitation and emission maximum. Atto 647 is characterized by a high photostability and brightness. In addition, this label has good water solubility and low triplet formation ability.

Applicazioni

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


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Articoli

Fluorescence lifetime measurement is advantageous over intensity-based measurements. Applications include fluorescence lifetime assays, sensing and FLI.

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