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85578

Spermidine trihydrochloride

Name: Spermidine trihydrochloride
Brand: SIGMA

BioXtra, ≥99.5% (AT)

Sinonimo/i:

N-(3-Aminopropyl)-1,4-butanediamine trihydrochloride

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Informazioni su questo articolo

Formula condensata:

NH₂(CH₂)₃NH(CH₂)₄NH₂ · 3HCl

Numero CAS:

334-50-9

Peso molecolare:

254.63

UNSPSC Code:

12352116

NACRES:

NA.26

PubChem Substance ID:

57653005

EC Number:

206-379-0

Beilstein/REAXYS Number:

3552356

MDL number:

MFCD00012918

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Proprietà

product line

BioXtra

Quality Segment

100

assay

≥99.0% (TLC), ≥99.5% (AT)

form

powder

impurities

insoluble matter, passes filter test

pH

3.0-6.0 (25 °C, 1 M in H₂O)

mp

257-259 °C (lit.)

solubility

H₂O: 1 M at 20 °C, clear, colorless

anion traces

sulfate (SO₄^2-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤50 mg/kg, Ni: ≤5 mg/kg, Pb: ≤10 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg

λ

1 M in H₂O

UV absorption

λ: 260 nm A_max: 0.2, λ: 280 nm A_max: 0.1

SMILES string

Cl[H].Cl[H].Cl[H].NCCCCNCCCN

InChI

1S/C7H19N3.3ClH/c8-4-1-2-6-10-7-3-5-9;;;/h10H,1-9H2;3*1H

InChI key

LCNBIHVSOPXFMR-UHFFFAOYSA-N

Descrizione

General description

Spermidine trihydrochloride is an aliphatic polyamine. Its crystalline structure has been studied. It has been reported to preserve the nuclei and prevent proteolytic degradation of histones during the macronuclei isolation from Tetrahymena pyriformis.[1]

Application

Spermidine trihydrochloride is used for the following applications:

DNA extraction and restriction endonuclease digestion[2]
Nuclear transplantation[3]
During the chemical analyses of the squid species Todaropsis eblanae[4]
As one of the agents in intercalating drug.[5]
Platelet aggregation[6]

It may be used in the synthesis of palladium complexes like [sperH₃]₂[PdCl₄]₃, [PdCl₂(sperH)]₂[PdCl₄], and [(PdCl₂)₃(sper)₂] by reacting with K₂PdCl₄ and PdCl₂.[7]

Biochem/physiol Actions

Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; Stimulates T₄ polynucleotide kinase activity.

Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; may be used for purification of DNA binding proteins. Stimulates T₄ polynucleotide kinase activity.

Other Notes

Buffer component for chromosome isolation in bivariate flow cytogenetic analysis and sorting[8].

Disclaimer

Solutions should be sterile-filtered, not autoclaved, if sterile solution is necessary. Spermidine deaminates with time; solutions should be stored frozen. Prepare new solutions frequently.

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Questo articolo	85580	S2501	56766-M
Sigma-Aldrich 85578 Spermidine trihydrochloride	SAFC 85580 Spermidine trihydrochloride	Sigma-Aldrich S2501 Spermidine trihydrochloride	Sigma-Aldrich 56766-M Spermidine, Trihydrochloride
assay ≥99.0% (TLC), ≥99.5% (AT)	assay ≥99.0% (AT)	assay ≥98% (TLC)	assay ≥98% (TLC)
Quality Level 100	Quality Level 200	Quality Level 200	Quality Level 100
form powder	form powder	form powder	form solid
solubility H₂O: 1 M at 20 °C, clear, colorless	solubility H₂O: 0.1 g/mL, clear, colorless	solubility water: 100 mg/mL, clear, colorless	solubility ethanol: soluble, water: soluble
cation traces Al: ≤5 mg/kg, Ba: ≤5 mg/kg, Ca: ≤10 mg/kg, Co: ≤5 mg/kg, Cu: ≤5 mg/kg, K: ≤50 mg/kg, Mg: ≤5 mg/kg, Mo: ≤5 mg/kg, Ni: ≤5 mg/kg, Sr: ≤5 mg/kg, As: ≤0.1 mg/kg, Cd: ≤5 mg/kg, Fe: ≤5 mg/kg, Mn: ≤5 mg/kg, Pb: ≤10 mg/kg, Bi: ≤5 mg/kg, Li: ≤5 mg/kg, Zn: ≤5 mg/kg, Cr: ≤5 mg/kg, Na: ≤50 mg/kg	cation traces -	cation traces -	cation traces -
mp 257-259 °C (lit.)	mp ~250 °C (dec.), 257-259 °C (lit.)	mp 257-259 °C (lit.)	mp -

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pictograms

GHS07

signalword

Warning

hcodes

H315,H319

pcodes

P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Classe di stoccaggio

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c